Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Complete genome sequence of a methicillin-resistant Staphylococcus schleiferi strain from canine otitis externa in Korea

  • Lee, Gi Yong (School of Bioresources and Bioscience, Chung-Ang University) ;
  • Yang, Soo-Jin (School of Bioresources and Bioscience, Chung-Ang University)
  • Received : 2019.08.21
  • Accepted : 2019.10.23
  • Published : 2020.01.31
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Abstract

The increase in canine skin and soft tissue infections, such as pyoderma and otitis, caused by Staphylococcus schleiferi strains, is of significant zoonotic concern. In this study, we report the first complete genome sequence for a methicillin-resistant clinical isolate of S. schleiferi (MRSS) designated as SS4, obtained from a dog with otitis externa, in Korea. The genome of SS4 strain was of 2,539,409 bp and presented high G+C content ratio (35.90%) with no plasmid. Comparative analysis of SS4 genome revealed that it is closely related to 2142-05 and 5909-02 strains isolated from the canine skin infections in the USA.

Keywords

Acknowledgement

This work was supported by the Cooperative Research Program for Agriculture Science & Technology Development (Grant No. PJ012811) funded by Rural Development Administration, Republic of Korea.

References

  1. Freney J, Brun Y, Bes M, Meugnier H, Grimont F, Grimont PA, Nervi C, Fleurette J. Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., two species from human clinical specimens. Int J Syst Bacteriol 1988;38:168-172. https://doi.org/10.1099/00207713-38-2-168
  2. Penna B, Varges R, Medeiros L, Martins GM, Martins RR, Lilenbaum W. Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa. Vet Dermatol 2010;21:292-296. https://doi.org/10.1111/j.1365-3164.2009.00842.x
  3. May ER, Kinyon JM, Noxon JO. Nasal carriage of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma or both. Vet Microbiol 2012;160:443-448. https://doi.org/10.1016/j.vetmic.2012.06.020
  4. Penna B, Mendes W, Rabello R, Lilenbaum W. Carriage of methicillin susceptible and resistant Staphylococcus schleiferi among dog with or without topic infections. Vet Microbiol 2013;162:298-299. https://doi.org/10.1016/j.vetmic.2012.08.022
  5. Cain CL, Morris DO, Rankin SC. Clinical characterization of Staphylococcus schleiferi infections and identification of risk factors for acquisition of oxacillin-resistant strains in dogs: 225 cases (2003-2009). J Am Vet Med Assoc 2011;239:1566-1573. https://doi.org/10.2460/javma.239.12.1566
  6. Lee GY, Lee HH, Hwang SY, Hong J, Lyoo KS, Yang SJ. Carriage of Staphylococcus schleiferi from canine otitis externa: antimicrobial resistance profiles and virulence factors associated with skin infection. J Vet Sci 2019;20:e6. https://doi.org/10.4142/jvs.2019.20.e6
  7. Hau SJ, Frana T, Sun J, Davies PR, Nicholson TL. Zinc resistance within swine-associated methicillinresistant Staphylococcus aureus isolates in the United States is associated with multilocus sequence type lineage. Appl Environ Microbiol 2017;83:83.
  8. Misic AM, Cain CL, Morris DO, Rankin SC, Beiting DP. Complete genome sequence and methylome of Staphylococcus schleiferi, an important cause of skin and ear infections in veterinary medicine. Genome Announc 2015;3:3.
  9. Sasaki T, Tsubakishita S, Kuwahara-Arai K, Matsuo M, Lu YJ, Tanaka Y, Hiramatsu K. Complete genome sequence of methicillin-resistant Staphylococcus schleiferi strain TSCC54 of canine origin. Genome Announc 2015;3:3.
  10. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008;9:75. https://doi.org/10.1186/1471-2164-9-75
  11. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007;57:81-91. https://doi.org/10.1099/ijs.0.64483-0
  12. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016;66:1100-1103. https://doi.org/10.1099/ijsem.0.000760
  13. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S, Holden MT, Fookes M, Falush D, Keane JA, Parkhill J. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015;31:3691-3693. https://doi.org/10.1093/bioinformatics/btv421
  14. Darling AC, Mau B, Blattner FR, Perna NT. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 2004;14:1394-1403. https://doi.org/10.1101/gr.2289704