Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Restoration of the inflammatory gene expression by horse oil in DNCB-treated mice skin

  • Lee, Jae-Chul (Department of Biochemistry, College of Veterinary Medicine, Jeju National University) ;
  • Park, Ga-Ryoung (Department of Biochemistry, College of Veterinary Medicine, Jeju National University) ;
  • Choi, Byoung-Soo (Department of Biochemistry, College of Veterinary Medicine, Jeju National University) ;
  • Lee, Youngjae (Department of Biochemistry, College of Veterinary Medicine, Jeju National University) ;
  • Han, Chang-Hoon (Department of Biochemistry, College of Veterinary Medicine, Jeju National University)
  • Received : 2019.08.06
  • Accepted : 2019.11.09
  • Published : 2020.01.31
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Abstract

The present study evaluated the anti-inflammatory effect of horse oil in 2, 4-dinitrochlorobenzene (DNCB)-treated BALB/c mice. After the application of DNCB, the mice showed atopic dermatitis symptoms, including severe erythema, hemorrhage, and erosion, whereas those symptoms were alleviated by treatment with horse oil. To explain the anti-dermatitis effect of horse oil, the gene expression levels in the healing process in dorsal skin were observed using a cDNA microarray. The cDNA microarray analysis revealed that the expression levels of 30 genes related to the inflammation, including Ccr1, Ccr2, Ccl20, Anxa1, and Hc genes, were up-regulated (higher than 2.0-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. In contrast, the gene expression levels of 28 genes related to inflammation, including chemokine genes Ccl5, Ccl7, Ccl8, Cxcl10, and Cxcl13 genes, were down-regulated (lower than 0.5-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. Overall, the results show that horse oil restores the expression levels of genes related to inflammation that were perturbed by DNCB treatment.

Keywords

Acknowledgement

This research was supported by a 2018 scientific promotion program funded by Jeju National University.

References

  1. Leung DY, Soter NA. Cellular and immunologic mechanisms in atopic dermatitis. J Am Acad Dermatol 2001;44:S1-S12. https://doi.org/10.1067/mjd.2001.109815
  2. Hanifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 1980;92:44-47.
  3. Leung DY. Atopic dermatitis: new insights and opportunities for therapeutic intervention. J Allergy Clin Immunol 2000;105:860-876. https://doi.org/10.1067/mai.2000.106484
  4. Joo HA, Bae HJ, Park MC, Beak SC, Hong SH, Yang HJ, Cho HW, Jung SY, Cho JH, Hwang CY. A Clinical Study for the effect of cosmetic cream containing Samwhangsejegamibang extracts on atopic dermatitis patients. J Korean Med Ophthalmol Otolaryngol Dermatol 2012;25:129-149. https://doi.org/10.6114/JKOOD.2012.25.3.129
  5. Pacor ML, Di Lorenzo G, Martinelli N, Mansueto P, Rini GB, Corrocher R. Comparing tacrolimus ointment and oral cyclosporine in adult patients affected by atopic dermatitis: a randomized study. Clin Exp Allergy 2004;34:639-645. https://doi.org/10.1111/j.1365-2222.2004.1907.x
  6. Tasaka K. Epinastine: an update of its pharmacology, metabolism, clinical efficacy and tolerability in the treatment of allergic diseases. Drugs Today (Barc) 2000;36:735-757. https://doi.org/10.1358/dot.2000.36.11.1136048
  7. Li SZ. Bencaogangmu. People's Medical Publishing House, Beijing, 1982.
  8. Tao HJ. Bencaojingjizhu. People's Medical Publishing House, Beijing, 1994.
  9. Wang B. Huangdineijing Lingshu. Chung Hwa Book Company, Taibei, 1972.
  10. Lee YS, Yoon JH, Kim BA, Park CI, Yoo WK, Cho JW, Kim MR. Effects of horse oil on the DNCB-induced contact hypersensitivity in BALB/c mice. Korean J Herbol 2013;28:77-81. https://doi.org/10.6116/kjh.2013.28.4.77
  11. Carbon S, Ireland A, Mungall CJ, Shu S, Marshall B, Lewis S; AmiGO Hub; Web Presence Working Group. AmiGO: online access to ontology and annotation data. Bioinformatics 2009;25:288-289. https://doi.org/10.1093/bioinformatics/btn615
  12. Vielhauer V, Berning E, Eis V, Kretzler M, Segerer S, Strutz F, Horuk R, Grone HJ, Schlondorff D, Anders HJ. CCR1 blockade reduces interstitial inflammation and fibrosis in mice with glomerulosclerosis and nephrotic syndrome. Kidney Int 2004;66:2264-2278. https://doi.org/10.1111/j.1523-1755.2004.66038.x
  13. Platt AM, Bain CC, Bordon Y, Sester DP, Mowat AM. An independent subset of TLR expressing CCR2-dependent macrophages promotes colonic inflammation. J Immunol 2010;184:6843-6854. https://doi.org/10.4049/jimmunol.0903987
  14. Uchida K, Koike Y, Hashimoto K, Saigusa S, Inoue M, Otake K, Tanaka K, Matsushita K, Okita Y, Fujikawa H, Araki T, Mohri Y, Kusunoki M. The increased expression of CCL20 and CCR6 in rectal mucosa correlated to severe inflammation in pediatric ulcerative colitis. Gastroenterol Res Pract 2015;2015:856532. https://doi.org/10.1155/2015/856532
  15. Furue M, Koga T, Yamashita N. Soluble E-selectin and eosinophil cationic protein are distinct serum markers that differentially represent clinical features of atopic dermatitis. Br J Dermatol 1999;140:67-72. https://doi.org/10.1046/j.1365-2133.1999.02609.x
  16. Segerer S, Djafarzadeh R, Grone HJ, Weingart C, Kerjaschki D, Weber C, Kungl AJ, Regele H, Proudfoot AE, Nelson PJ. Selective binding and presentation of CCL5 by discrete tissue microenvironments during renal inflammation. J Am Soc Nephrol 2007;18:1835-1844. https://doi.org/10.1681/ASN.2006080837
  17. Akdis M, Akdis CA, Weigl L, Disch R, Blaser K. Skin-homing, CLA+ memory T cells are activated in atopic dermatitis and regulate IgE by an IL-13-dominated cytokine pattern: IgG4 counter-regulation by CLAmemory T cells. J Immunol 1997;159:4611-4619.
  18. Asano K, Takahashi N, Ushiki M, Monya M, Aihara F, Kuboki E, Moriyama S, Iida M, Kitamura H, Qiu CH, Watanabe T, Tanaka M. Intestinal CD169+ macrophages initiate mucosal inflammation by secreting CCL8 that recruits inflammatory monocytes. Nat Commun 2015;6:7802. https://doi.org/10.1038/ncomms8802
  19. Choi JY, Choi B, Shim JA, Lee ES, Kim DY, Bang D, Sohn S. IL-2/IL-2 antibody immune complex regulates HSV-induced inflammation through induction of IL-2 receptor alpha, beta, and gamma in a mouse model. Immunobiology 2015;220:1381-1392. https://doi.org/10.1016/j.imbio.2015.07.004