The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Grovesinia moricola Causing Zonate Leaf Spots on Lespedeza cyrtobotrya in Korea

참싸리 겹둥근무늬병균 Grovesinia moricola 동정

  • Park, Ji-Hyun (Division of Forest Insect Pests and Diseases, National Institute of Forest Science) ;
  • Jung, Bok-Nam (Division of Forest Insect Pests and Diseases, National Institute of Forest Science) ;
  • Lee, Sang-Hyun (Division of Forest Insect Pests and Diseases, National Institute of Forest Science) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 박지현 (국립산림과학원 산림병해충연구과) ;
  • 정복남 (국립산림과학원 산림병해충연구과) ;
  • 이상현 (국립산림과학원 산림병해충연구과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2019.11.25
  • Accepted : 2019.12.16
  • Published : 2020.03.31
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Abstract

In September 2017, a heavy damage by premature defoliation with the zonate leaf spots was observed in several shrubs of Lespedeza cyrtobotrya growing at Mt. Obongsan in Chuncheon, Korea. Numerous cone-shaped, white sporophores of a fungus were observed on lesions of the abaxial leaf surface. A similar fungus was isolated in September 2019 from the leaves of L. cyrtobotrya growing at Mt. Taegisan in Hoengseong, Korea. The morphological characteristics of the sporophores were consistent with those of Grovesinia moricola. The species identification was confirmed by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA from the two isolates (KACC48417 and KACC48934). The fungal pathogenicity was determined by an artificial inoculation in conditions of relative humidity and temperature of 100% and 15±2℃, respectively. This is the first report of association of G. moricola with L. cyrtobotrya in Korea.

2017년 9월 춘천의 오봉산에서 참싸리 잎에 겹둥근무늬가 생기고 조기낙엽이 일어나는 등 큰 피해가 발견되었다. 병든 잎의 뒷면에는 원뿔형의 흰색 포자과가 다수 발견되었다. 2019년 9월 횡성의 태기산에서도 참싸리 잎에서 유사한 증상이 발견되었다. 이러한 증상에서 발견된 포자과의 형태적 특징으로 보아 이 곰팡이는 Grovesinia moricola와 일치하였다. 확보된 두 균주(KACC48417 및 KACC48934)의 ITS rDNA 염기서열 분석을 통해 동정을 확인하였다. 이 곰팡이의 병원성은 상대습도 100%와 15±2℃의 조건에서 인공접종을 통하여 확인하였다. 이는 한국에서 참싸리와 G. moricola의 관계를 연구한 최초의 보고이다.

Keywords

References

  1. Wu Z, Raven PH, Hong D. Missouri Botanical Garden. Flora of China [Internet]. Beijing: Science Press; 2008.
  2. Yu BD, Shim SR. The optimal seeding quantity of Lespedeza cyrtobotrya Miquel and Indigofera pseudo-tinctoria Matsumura as leguminous woody plants for the cut-slope revegetation. J Korean Env Res Tech 2016;19:61-71.
  3. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  4. Noro S, Sugiyama S, Matsunaka K, Harada Y. Perfect stage of Cristularialla moricola and its host plants in Japan. Ann Phytopath Soc Japan 1986;52:539.
  5. Hirano K, Iida W. Zonate leaf spot disease of crapemyrtle caused by Cristulariella pyramidalis Waterman and Marshall. Tech Bull Fac Hort Chiba Univ 1975;23:53-61.
  6. Cho SE, Park JH, Lee SH, Lee HB, Shin HD. Zonate leaf spot of Acer negundo caused by Cristulariella moricola in Korea. Res Plant Dis 2012;18:45-8. https://doi.org/10.5423/RPD.2012.18.1.045
  7. Cho SE, Hong SB, Choi IY, Oh HT, Shin HD. First report of zonate leaf spot caused by Grovesinia moricola on adzuki bean in Korea. Plant Dis 2017;101:1677.
  8. Shin HD, Choi YJ, Hong SH, Lee YH. Grovesinia moricola occurring on Parthenocissus tricuspidata. Korean J Mycol 2019;47:271-4. https://doi.org/10.4489/KJM.20190031
  9. Su HJ, Leu SC. Zonate leaf spot of Indian jujube caused by Cristulariella moricola. Plant Dis 1983;67:915-6. https://doi.org/10.1094/PD-67-915
  10. Lee SB, Taylor JW. Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press, Inc; 1990. p. 282-7.
  11. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press, Inc; 1990. p. 315-22.
  12. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  13. Narumi-Saito T, Hosoya T, Sano T, Harada Y. Nervostroma, gen. nov. in the Sclerotiniaceae, the teleomorph of Cristulariella , and Hinomyces anam. gen. nov. to accommodate the anamorph of Grovesinia: Reassessment of the genus Cristulariella. Mycoscience 2006;47:351-9. https://doi.org/10.1007/S10267-006-0313-9
  14. Johnston PR, Seifert KA, Stone JK, Rossman AY, Marvanova L. Recommendations on generic names competing for use in Leotiomycetes (Ascomycota). IMA Fungus 2014;5:91-120. https://doi.org/10.5598/imafungus.2014.05.01.11
  15. La YJ, Woo KS, Lee KJ. Diseases, insect pests, and abiotic disorders of landscape trees in Korea. Seoul National University Press; 2009.