Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antioxidant and Anti-Obesity Activities of Polygonum cuspidatum Extract through Alleviation of Lipid Accumulation on 3T3-L1 Adipocytes

  • Choi, Da-Hye (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Han, Joon-Hee (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Yu, Keun-Hyung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Hong, Min (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Lee, Sun-Yeop (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Park, Ka-Hee (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Lee, Soo-Ung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF)) ;
  • Kwon, Tae-Hyung (Department of Research and Development, Chuncheon Bio-industry Foundation (CBF))
  • Received : 2019.10.18
  • Accepted : 2019.12.04
  • Published : 2020.01.28
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Abstract

Natural products are widely used due to their various biological activities which include anti-inflammatory, antioxidant, and anti-obesity effects. In this study, we determined the antioxidative and anti-obesity effects of Polygonum cuspidatum 50% ethanol extract (PEE). The antioxidative effect of PEE was evaluated using its radical scavenging activity, total phenolic content, and reducing power. The anti-obesity effect of PEE was investigated using 3T3-L1 adipocytes. The antioxidative activity of PEE was progressively increased in various concentrations, mainly due to the presence of phenolic compounds. PEE also alleviated lipid accumulation on 3T3-L1 adipocytes and downregulated the mRNA and protein production of adipogenesis-related (SREBP-1c, PPARγ, C/EBPα) and lipogenesis-related (aP2, FAS, ACC) markers. Furthermore, we found that the inhibitory effect on lipid accumulation via PEE was caused by the alleviation of NF-κB, p38 MAPK, ERK1/2, and JNK at the protein level. Taken together, our results imply that PEE is a potential antioxidant that can prevent obesity-associated disorders.

Keywords

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