Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

  • Lee, Nan-Yeong (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Choi, Doo-Ho (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Mi-Gyeong (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Jeong, Min-Ji (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kwon, Hae-Jun (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Dong-Hyun (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Young-Guk (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Luccio, Eric di (School of Life Science, College of Natural Sciences, Kyungpook National University) ;
  • Arioka, Manabu (Department of Biotechnology, The University of Tokyo) ;
  • Yoon, Hyeok-Jun (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University)
  • Received : 2020.01.29
  • Accepted : 2020.02.16
  • Published : 2020.02.28
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Abstract

Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)-1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)-1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.

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