한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제35권1호
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  • Pages.94-101
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  • 2020
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Study on the In-vitro Culture Method for Normal Embryonic Cell Development of Porcine Parthenogenetic Embryos

  • Jung, Na-Hyeon (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Kim, Dae-Seung (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2020.03.12
  • 심사 : 2020.03.16
  • 발행 : 2020.03.31
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초록

In the early development of parthenogenetic embryo, cytoplasm and nucleic acid fragmentation may be a cause of lower embryo development. The purpose of this study was to evaluate whether embryonic development and apoptosis factors can be reduced by controlling the in-vitro culture environment by the addition of hormones, pregnancy serum and uterine milk. Our study showed that the activity of Casp-3 increased within the cytoplasm when artificially used hormones to induce the incubation environment, and PCNA's manifestation was low. However, the addition of pregnant serum appeared to lower the Casp-3 activity compared to the other groups. In addition, MMP-9 activity was increased and early embryo development and cytoplasmic fidelity were also increased. Therefore, the results of the present study showed that the use of gestational serum in the development of parthenogenetic embryo inhibit apoptosis and increases cytoplasmic reorganization by natural environmental control in in vitro culture.

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참고문헌

  1. Alikani M, Cohen J, Tomkin G, Garrisi GJ, Mack C, Scott RT. 1999. Human embryo fragmentation in vitro and its implications for pregnancy and implantation. Fertil. Steril. 71:836-842. https://doi.org/10.1016/S0015-0282(99)00092-8
  2. Azuma T, Kondo T, Ikeda S, Imai H, Yamada M. 2002. Effects of EDTA saturated with Ca2+ (Ca-EDTA) on pig, bovine and mouse oocytes at the germinal vesicle stage during maturation culture and the involvement of chelation of Zn2+ in pronuclear formation induction by Ca-EDTA. Reproduction 124:235-240. https://doi.org/10.1530/reprod/124.2.235
  3. Bae HK, Kim SH, Lee SY, Hwang IS, Park CK, Yang BK, Cheong HT. 2013. Effect of antioxidant treatment during parthenogenetic activation procedure on the reactive oxygen species levels and development of the porcine parthenogenetic embryos. Reprod. Dev. Biol. 37:51-55. https://doi.org/10.12749/RDB.2013.37.1.51
  4. Bing Y, Che L, Hirao Y, Takenouchi N, Rodriguez-Martinez H, Nagai T. 2003. Parthenogenetic activation and subsequent development of porcine oocytes activated by a combined electric pulse and butyrolactone I treatment. J. Reprod. Dev. 49:159-166. https://doi.org/10.1262/jrd.49.159
  5. Contro V, Basile JR, Proia P. 2015. Sex steroid hormone receptors, their ligands, and nuclear and non-nuclear pathways. AIMS Mol. Sci. 2:294-310. https://doi.org/10.3934/molsci.2015.3.294
  6. Costa R, Carneiro BA, Tavora F, Pai SG, Kaplan JB, Chae YK, Chandra S, Kopp PA, Giles FJ. 2016. The challenge of developmental therapeutics for adrenocortical carcinoma. Oncotarget 7:46734-46749. https://doi.org/10.18632/oncotarget.8774
  7. Diao YF, Kenji N, Han RX, Lin T, Oqani RK, Kang JW, Jin DI. 2013b. Effects of trichostatin A on in vitro development of porcine parthenogenetic and nuclear transfer embryos. Reprod. Dev. Biol. 37:57-64. https://doi.org/10.12749/RDB.2013.37.2.57
  8. Diao YF, Naruse KJ, Han RX, Li XX, Oqani RK, Lin T, Jin DI. 2013a. Treatment of fetal fibroblasts with DNA methylation inhibitors and/or histone deacetylase inhibitors improves the development of porcine nuclear transfer-derived embryos. Anim. Reprod. Sci. 141:164-171. https://doi.org/10.1016/j.anireprosci.2013.08.008
  9. Hafez ESE and Hafez B. 2006. Reproduction in farm animals. 7th ed, Wiley-Blackwell, Ames, pp. 68-81.
  10. Hajjari M and Jahani MM. 2014. H19: a long non-coding RNA with different roles in cancer progression. Gene Ther. Mol. Biol. 16:172-181.
  11. Kaufman MH. 1975. The experimental induction of parthenogenesis in the mouse. In: Balls M, Wild AE (Eds.), The Early Development of Mammals: the Second Symposium of the British Society for Developmental Biology. Cambridge University Press, Cambridge, pp. 25-44.
  12. Kim SH and Yoon JT. 2019. Effect of serum-containing and serum-free culture medium-mediated activation of matrix metalloproteinases on embryonic developmental competence. Czech J. Anim. Sci. 64:473-482. https://doi.org/10.17221/205/2019-CJAS
  13. Lee SE, Hwang KC, Sun SC, Xu YN, Kim NH. 2011. Modulation of autophagy influences development and apoptosis in mouse embryos developing in vitro. Mol. Reprod. Dev. 78:498-509. https://doi.org/10.1002/mrd.21331
  14. Ma SF, Liu XY, Miao DQ, Han ZB, Zhang X, Miao YL, Yanagimachi R, Tan JH. 2005. Parthenogenetic activation of mouse oocytes by strontium chloride: a search for the best conditions. Theriogenology 64:1142-1157. https://doi.org/10.1016/j.theriogenology.2005.03.002
  15. Marques MG, Nicacio AC, de Oliveira VP, Nascimento AB, Caetano HV, Mendes CM, Mello MR, Milazzotto MP, Assumpcao ME, Visintin JA. 2007. In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Anim. Reprod. Sci. 97:375-381. https://doi.org/10.1016/j.anireprosci.2006.02.013
  16. Mateusen B, Van Soom A, Maes DG, Donnay I, Duchateau L, Lequarre AS. 2005. Porcine embryo development and fragmentation and their relation to apoptotic markers: a cinematographic and confocal laser scanning microscopic study. Reproduction 129:443-452. https://doi.org/10.1530/rep.1.00533
  17. McCullough LE, Eng SM, Bradshaw PT, Cleveland RJ, Teitelbaum SL, Neugut AI, Gammon MD. 2012. Fat or fit: the joint effects of physical activity, weight gain, and body size on breast cancer risk. Cancer 118:4860-4868. https://doi.org/10.1002/cncr.27433
  18. Moon HJ, Shim JH, Hwang IS, Park MR, Kim DH, Ko YG, Park CK, Im GS. 2009. Effects of FBS(fetal bovine serum) and pFF(porcine follicular fluid) on in vitro maturation and development of porcine parthenogenetic and nuclear transfer embryos. Reprod. Dev. Biol. 33:85-91.
  19. Park JH, Kwon DJ, Lee BK, Hwang IS, Park CK, Yang BK, Cheong HT. 2009. Comparison of microtubule distributions between somatic cell nuclear transfer and parthenogenetic porcine embryos. Reprod. Dev. Biol. 33:13-18.
  20. Park MR, Im JH, Chung HJ, Kim KW, Byun SJ, Hwang SS, Im GS. 2016. Effects of CD26 in parthenogenetically activated porcine embryos. J. Embryo Transf. 31:319-322. https://doi.org/10.12750/JET.2016.31.4.319
  21. Pawar S, Hantak AM, Bagchi IC, Bagchi MK. 2014. Minireview: steroid-regulated paracrine mechanisms controlling implantation. Mol. Endocrinol. 28:1408-1422. https://doi.org/10.1210/me.2014-1074
  22. Petters RM and Wells KD. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl. 48:61-73.
  23. Presicce GA and Yang X. 1994. Parthenogenetic development of bovine oocytes matured in vitro for 24 hr and activated by ethanol and cycloheximide. Mol. Reprod. Dev. 38:380-385. https://doi.org/10.1002/mrd.1080380405
  24. Rossant J and Tam PPL. 2017. New insights into early human development: lessons for stem cell derivation and differentiation. Cell Stem Cell 20:18-28. https://doi.org/10.1016/j.stem.2016.12.004
  25. Son JM, Lee DS, Lee ES, Cho JK, Shin ST. 2008. Effect of porcine serum as macromolecule on the meiotic maturation and embryonic development of porcine oocytes. J. Embryo Transf. 23:93-100.
  26. Son JY, Kim SH, Jung DW, Ryu CY, Yoon JT. 2015. Expression analysis of programmed cell death genes in porcine parthenogenesis. J. Embryo Transf. 30:239-248. https://doi.org/10.12750/JET.2015.30.3.239
  27. Won CH, Park SK, Kim KY, Roh SH. 2008. Existence of amino acids in defined culture medium influences in vitro development of parthenogenetic and nuclear transfer porcine embryos. J. Embryo Transf. 23:245-250.
  28. Yeo JH, Hwang IS, Park JK, Kwon DJ, Im S, Park EW, Lee JW, Park CK, Hwang S. 2014. Transgenic efficiency of FoxN1-targeted pig parthenogenetic embryos. J. Embryo Transf. 29:339-344. https://doi.org/10.12750/JET.2014.29.4.339
  29. Yi YJ and Park CS. 2005. Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine. Anim. Reprod. Sci. 86:297-304. https://doi.org/10.1016/j.anireprosci.2004.07.007
  30. Zeyneloglu H, Kahraman S, Pirkevi C. 2011. Co-culture techniques in assisted reproduction: history, advances and the future. J. Reprod. Stem Cell Biotechnol. 2:29-40. https://doi.org/10.1177/205891581100200104
  31. Zhao J, Ross JW, Hao Y, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. 2009. Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer. Biol. Reprod. 81:525-530. https://doi.org/10.1095/biolreprod.109.077016