Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Anti-inflammation on Oryeong-san Formulation for Mix Extract Tablet

오령산 정제 개발 및 항염증 효과

  • Kim, Se Jin (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Leem, Hyun Hee (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Nam, Won Hee (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Son, Su Mi (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Choi, Hye Min (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Kim, Myung Jin (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Kim, Jung Ok (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development) ;
  • Lee, Hwa Dong (Korean Medicine R&D Team 2, National Institute for Korean Medicine Development)
  • 김세진 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 임현희 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 남원희 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 손수미 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 최혜민 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 김명진 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 김정옥 (한국한의약진흥원 한의기술 R&D 2팀) ;
  • 이화동 (한국한의약진흥원 한의기술 R&D 2팀)
  • Received : 2020.10.12
  • Accepted : 2020.12.11
  • Published : 2020.12.25
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Abstract

Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.

Keywords

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