Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Changes in Cerebral Blood flow Following Fermented Garlic Extract Solution with High Content of Nitrite

흰쥐에서 고용량 아질산이온 함유 마늘 발효농축액에 의한 뇌혈류 변화

  • Yu, Hyeok (Department of Emergency Medicine, Wonkwang University School of Medicine) ;
  • Rong, Zhang Xiao (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University) ;
  • Koo, Ho (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University) ;
  • Chun, Hyun Soo (Humanenos Co. LTD) ;
  • Yoo, Su Jin (Department of Emergency Medicine, Wonkwang University School of Medicine) ;
  • Kim, Min Sun (Center for Nitric Oxide Metabolite at Wonkwang University & Department of Physiology, Wonkwang University)
  • 유혁 (원광대학교 의과대학 응급의학교실) ;
  • ;
  • 구호 (원광대학교 산화질소 대사체 연구소 & 의과대학 생리학교실) ;
  • 천현수 ((유)휴먼에노스) ;
  • 유수진 (원광대학교 의과대학 응급의학교실) ;
  • 김민선 (원광대학교 산화질소 대사체 연구소 & 의과대학 생리학교실)
  • Received : 2020.09.22
  • Accepted : 2020.10.30
  • Published : 2020.12.25
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Abstract

Nitrate-nitrite-nitric oxide (NO) pathway is a major alternative source of NO and is essential for NO - dependent physiological functions in body. Food supplements having nitrate/nitrite can improve metabolic syndromes including hypertension through antioxidant activity or vasodilation. The purpose of this study was to observe the effects of fermented garlic (F. garlic) having high concentration of NO2- on changes in blood flow and nitric oxide synthesis in the cerebral cortex of rodents. The generation of nitric oxide detected by a chemi-luminescence detector was higher in F. Garlic compared with NaNO2 solution under artificial gastric juice with pH 2.0. Ether F. garlic or NaNO2 diluted with artificial cerebrospinal fluid was directly applied into around the needle probe of laser Doppler flow meter that was located on epidural surface of the cortex. Direct application of F. garlic resulted in increase of cerebral blood flow detected by a laser Doppler flow meter with a dose-dependent manner. Compared with NaNO2 solution, F. garlic produced changes in cerebral blood flow at lower concentration of NO2-. Pretreatment of methylene blue, a guanylyl cyclase inhibitor prevented upregulation of cerebral blood flow by the treatment of F. garlic. In addition, the application of F. garlic with 250, 500ppm of NO2- caused significantly the production of NO in the cortical tissue but NaNO2 solution with 500ppm of NO2- did not. In summary, these results suggested that F. garlic with high content of NO2- induce increase in cerebral blood flow through nitric oxide-dependent signal pathway.

Keywords

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