Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Determination of the Flavonoids and Limonoids in Citrus junos Seed Shells Using a UPLC-DAD-ESI/MS

  • Jo, Ara (College of Pharmacy, Sunchon National University) ;
  • Shin, Ji hun (College of Pharmacy, Sunchon National University) ;
  • Song, Hwa young (College of Pharmacy, Sunchon National University) ;
  • Lee, Ye Eun (College of Pharmacy, Sunchon National University) ;
  • Jeong, Da Eun (College of Pharmacy, Sunchon National University) ;
  • Oh, Sung Hwa (Nano Bio Research Center, JBF) ;
  • Mun, Myung Jae (Nano Bio Research Center, JBF) ;
  • Lee, Mina (College of Pharmacy, Sunchon National University)
  • Received : 2019.10.29
  • Accepted : 2019.12.21
  • Published : 2020.03.31
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Abstract

Citrus junos seeds (CS) have been traditionally used for the treatment of cancer and neuralgia. They are also used to manufacture edible oil and cosmetic perfume. A large amount of CS shells without oil (CSS) are discarded after the oil in CS is used as foods or herbal remedy. To efficiently utilize CSS as a by-products, it needs to be studied through chemical analysis. Therefore, we developed an ultra-performance liquid chromatography (UPLC)-diode array detection (DAD) method for simultaneous determination and quantitative analysis of five components (two flavonoids and threes limonoids) in CSS. A Waters Acquity UPLC HSS T3 column C18 (2.1 × 100 mm, 1.8 ㎛) was used for this separation. It was maintained at 40 ℃. The mobile phase used for the analysis was distilled water and acetonitrile with gradient elution. To identify the quantity of the five components, a mass spectrometer (MS) with an electrospray ionization (ESI) source was used. The regression equation showed great linearity, with correlation coefficient ≥ 0.9912. Limits of detection (LOD) and limits of quantification (LOQ) of the five compounds were 0.09 - 0.13 and 0.26 - 0.38 ㎍/mL, respectively. Recoveries of extraction ranged from 97.45% to 101.91%. Relative standard deviation (RSD) values of intra- and inter-day precision were 0.06 - 1.15% and 0.19 - 0.25%, respectively. This UPLC-DAD method can be validated to simultaneously analyze quantities of marker flavonoids and limonoids in CSS.

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References

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