DOI QR코드

DOI QR Code

Soluble fraction from silk mat induced bone morphogenic protein in RAW264.7 cells

  • Kim, Seong-Gon (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Jo, You-Young (Sericultural and Apicultural Division, National Institute of Agricultural Science, RDA) ;
  • Kweon, HaeYong (Sericultural and Apicultural Division, National Institute of Agricultural Science, RDA)
  • Received : 2020.11.30
  • Accepted : 2020.12.17
  • Published : 2020.12.31

Abstract

The objective of this study was to evaluate the changes in gene expression after incubation of cells with soluble fraction from different silk mat layers. A silk cocoon from Bombyx mori was separated into 4 layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to outer layer). Each silk mat was placed into normal saline and collected soluble fraction. They were administered to RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis. Layer 1 and 4 groups showed significantly higher expression of BMP-2 at 8 h after administration of soluble fraction (P < 0.05). Runx2 expression was significantly higher in Layer 4 group at 8h (P < 0.05). The silk mat from the innermost and outermost portion of the silkworm cocoon showed a significant change in the expression of genes that are associated with osteoinduction such as BMP-2 and runx2.

Keywords

References

  1. Ahmad P, Della Bella E, Stoddart MJ (2020) Applications of bone morphogenetic proteins in dentistry: A bibliometric analysis. Biomed Res Int 2020, 5971268.
  2. Edelmayer M, Wehner C, Ulm C, Zechner W, Shafer D, Agis H (2020) Which substances loaded onto collagen scaffolds influence oral tissue regeneration?-an overview of the last 15 years. Clin Oral Investig 24(10), 3363-3394. https://doi.org/10.1007/s00784-020-03520-0
  3. El Bialy I, Jiskoot W, Reza Nejadnik M (2017) Formulation, delivery and stability of bone morphogenetic proteins for effective bone regeneration. Pharm Res 34(6), 1152-1170. https://doi.org/10.1007/s11095-017-2147-x
  4. Jo YY, Kim DW, Choi JY, Kim SG (2019) 4-Hexylresorcinol and silk sericin increase the expression of vascular endothelial growth factor via different pathways. Sci Rep 9, 3448. https://doi.org/10.1038/s41598-019-40027-5
  5. Jo YY, Kweon H, Kim DW, Baek K, Kim MK, Kim SG, et al. (2017) Bone regeneration is associated with the concentration of tumour necrosis factor-α induced by sericin released from a silk mat. Sci Rep 7, 15589. https://doi.org/10.1038/s41598-017-15687-w
  6. Kaur J, Rajkhowa R, Tsuzuki T, Millington K, Zhang J, Wang X (2013) Photoprotection by silk cocoons. Biomacromolecules 14(10), 3660-3667. https://doi.org/10.1021/bm401023h
  7. Kim DW, Jo YY, Garagiola U, Choi JY, Kang YJ, Oh JH, et al. (2020) Increased level of vascular endothelial growth factors by 4-hexylresorcinol is mediated by transforming growth factor-β1 and accelerates capillary regeneration in the burns in diabetic animals. Int J Mol Sci 21(10), 3473. https://doi.org/10.3390/ijms21103473
  8. Kim JW, Jo YY, Kim JY, Oh J, Yang BE, Kim SG (2019a) Clinical study for silk mat application into extraction socket: a split-mouth, randomized clinical trial. Appl Sci 9(6), 1208. https://doi.org/10.3390/app9061208
  9. Kim JW, Jo YY, Kim JY, Oh J, Yang BE, Kim SG (2019b) Clinical study for silk mat application into extraction socket: a split-mouth, randomized clinical trial. Maxillofac Plast Reconstr Surg 41, 16. https://doi.org/10.1186/s40902-019-0199-z
  10. Kim JW, Jo YY, Kweon HY, Kim DW, Kim SG (2018) The effects of proteins released from silk mat layers on macrophages. Maxillofac Plast Reconstr Surg 40, 10. https://doi.org/10.1186/s40902-018-0149-1
  11. Kim SG (2020) Immunomodulation for maxillofacial reconstructive surgery. Maxillofac Plast Reconstr Surg 42(1), 5. https://doi.org/10.1186/s40902-020-00249-4
  12. Kim SG, Jeong JH, Che X, Park YT, Lee SW, Jung ES, et al. (2013) Reconstruction of radial bone defect using gelatin sponge and a BMP-2 combination graft. BMB Rep 46(6), 328-333. https://doi.org/10.5483/BMBRep.2013.46.6.231
  13. Kweon HY, Jo YY, Seok H, Kim SG, Chae WS, Sapru S, et al. (2017) In vivo bone regeneration ability of different layers of natural silk cocoon processed using an eco-friendly method. Macromol Res 25, 806-816. https://doi.org/10.1007/s13233-017-5085-x
  14. Lee SW, Hahn BD, Kang TY, Lee MJ, Choi JY, Kim MK, Kim SG (2014) Hydroxyapatite and collagen combination-coated dental implants display better bone formation in the peri-implant area than the same combination plus bone morphogenetic protein-2-coated implants, hydroxyapatite only coated implants, and uncoated implants. J Oral Maxillofac Surg 72(1), 53-60. https://doi.org/10.1016/j.joms.2013.08.031
  15. Mandal BB, Priya AS, Kundu S (2009) Novel silk sericin/gelatin 3-D scaffolds and 2-D films: fabrication and characterization for potential tissue engineering applications. Acta Biomater 5, 3007-3020. https://doi.org/10.1016/j.actbio.2009.03.026
  16. Medikeri RS, Meharwade VV, Sinha KA (2019) Effects of recombinant human bone morphogenetic protein-2 compared to other biomaterials in the treatment of intrabony defects in periodontitis patients: A systematic review. J Indian Soc Periodontol 23(4), 311-315. https://doi.org/10.4103/jisp.jisp_748_18
  17. Park CJ, Um IC (2018) Effect of heat treatment on the structural characteristics and properties of silk sericin film. Int J Indust Entomol 37, 36-42. https://doi.org/10.7852/ijie.2018.37.2.36
  18. Zhang Y, Zhao P, Dong Z, Wang D, Guo P, Guo X, et al. (2015) Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori. PLoS ONE 10, e0123403. https://doi.org/10.1371/journal.pone.0123403
  19. Zhou Q, Gu X, Dong J, Zhu C, Cai Z, He D, et al. (2019) The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment. Artif Cells Nanomed Biotechnol 47(1), 3329-3337. https://doi.org/10.1080/21691401.2019.1626867