한국식생활문화학회지 (Journal of the Korean Society of Food Culture)

  • 제35권6호
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  • Pages.597-604
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  • 2020
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  • 1225-7060(pISSN)
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  • 2288-7148(eISSN)
한국식생활문화학회 (Korean Society of Food Culture)
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산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능

Antioxidant and Hepatoprotective Activities of Bitter Melon (Momordica charantia Linn.) Leaves against Oxidative Stress

  • 전아영 (경성대학교 식품생명공학과 대학원) ;
  • 천원영 (경성대학교 식품생명공학과 대학원) ;
  • 윤지민 (경성대학교 식품생명공학과 대학원) ;
  • 김대중 (국립농산물품질관리원 시험연구소) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Jeon, Ahyeong (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Cheon, Wonyoung (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Yoon, Jimin (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Kim, Dae-Jung (Experiment research institute, National Agricultural Products Quality Management Service) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 투고 : 2020.11.20
  • 심사 : 2020.12.15
  • 발행 : 2020.12.31
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초록

This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.

키워드

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