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Occurrence and Characterization of Leaf Spot Caused by Septoria melissae on Lemon Balm in Korea

  • Yang, Seon-Ah (Department of Agricultural Biology, Jeonbuk National University) ;
  • Choi, In-Young (Department of Agricultural Biology, Jeonbuk National University) ;
  • Ju, Ho-Jong (Department of Agricultural Biology, Jeonbuk National University) ;
  • Lee, Kui-Jae (Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University) ;
  • Galea, Victor (School of Agriculture and Food Sciences, The University of Queensland) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 투고 : 2020.04.06
  • 심사 : 2020.09.28
  • 발행 : 2020.12.31

초록

Leaf spot on lemon balm is frequently observed in Korea, causing considerable damage to crops. In 2014 and 2015, the occurrence of leaf spot was observed in several production greenhouses at Suwon, Gongju, and Namwon in Korea. Symptoms on lower leaves initially developed as small, distinct, discolored lesions, which enlarged progressively turning into dark brown, angular spots surrounded by purplish-brown margins. Based on the morphological characteristics and sequence analysis of actin (ACT), translation elongation factor 1-alpha (EF-1α), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II second largest subunit (RPB2), the fungus associated with the lemon balm leaf spot was determined as Septoria melissae. To the best of our knowledge, this is the first report of lemon balm leaf spot caused by S. melissae in Asia as well as in Korea.

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참고문헌

  1. Dastmalchi K, Dorman HD, Oinonen PP, et al. Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract. LWT - Food Sci Technol. 2008;41(3):391-400. https://doi.org/10.1016/j.lwt.2007.03.007
  2. Farr DF, Rossman AY. Fungal databases, systematic mycology & microbiology laboratory. ARS, USDA [cited 2020 Mar 28]. Available from: http://nt.ars-grin.gov/fungaldatabases/.
  3. Quaedvlieg W, Verkley GJM, Shin HD, et al. Sizing up Septoria. Stud Mycol. 2013;75(1):307-390. https://doi.org/10.3114/sim0017
  4. Bakhshi M, Arzanlou M, Zare R, et al. New species of Septoria associated with leaf spot diseases in Iran. Mycologia. 2019;111(6):1056-1071. https://doi.org/10.1080/00275514.2019.1669376
  5. Verkley GJM, Quaedvlieg W, Shin HD, et al. A new approach to species delimitation in Septoria. Stud Mycol. 2013;75(1):213-305. https://doi.org/10.3114/sim0018
  6. Crous PW, Kang JC, Braun U. A phylogenetic redefinition of anamorph genera in Mycosphaerella based on ITS rDNA sequence and morphology. Mycologia. 2001;93(6):1081-1101. https://doi.org/10.2307/3761670
  7. Crous P, Summerell B, Carnegie A, et al. Unravelling Mycosphaerella: do you believe in genera? Persoonia. 2009;23:99-118. https://doi.org/10.3767/003158509X479487
  8. Verkley G, Starink-Willemse M. A phylogenetic study of some Septoria species pathogenic to Asteraceae based on ITS ribosomal DNA sequences. Mycol Progress. 2004;3(4):315-323. https://doi.org/10.1007/s11557-006-0101-0
  9. Verkley G, Starink-Willemse M, van Iperen A, et al. Phylogenetic analyses of Septoria species based on the ITS and LSU-D2 regions of nuclear ribosomal DNA. Mycologia. 2004;96(3):558-571. https://doi.org/10.2307/3762175
  10. Groenewald JZ, Nakashima C, Nishikawa J, et al. Species concepts in Cercospora: spotting the weeds among the roses. Stud Mycol. 2013;75(1):115-170. https://doi.org/10.3114/sim0012
  11. Golmohammadi R, Babai-Ahari A, Arzanlou M, et al. Morphological and molecular identification and pathogenicity of Septoria species involved in leaf spot disease on Populus species in East Azerbaijan, West Azerbaijan and Ardabil provinces. Iran J Plant Pathol. 2015;51(3):357-365.
  12. Bakhshi M, Arzanlou M. Multigene phylogeny reveals a new species and novel records and hosts in the genus Ramularia from Iran. Mycol Progress. 2017;16(7):703-712. https://doi.org/10.1007/s11557-017-1308-y
  13. Bakhshi M, Arzanlou M. Multigene phylogeny and morphotaxonony of Septoria spp. from Iran along with a checklist of Septoria-like taxa. Rostaniha. 2018;18:122-141.
  14. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. 1999;91(3):553-556. https://doi.org/10.2307/3761358
  15. O'Donnell K, Kistler HC, Cigelnik E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA. 1998;95(5):2044-2049. https://doi.org/10.1073/pnas.95.5.2044
  16. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocol. 1990;18:315-322.
  17. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol. 1990;172(8):4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  18. Quaedvlieg W, Kema GHJ, Groenewald JZ, et al. Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia. 2011;26:57-69. https://doi.org/10.3767/003158511X571841
  19. Hunter GC, Wingfield BD, Crous PW, et al. A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves. Stud Mycol. 2006;55:147-161. https://doi.org/10.3114/sim.55.1.147
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  21. Radulescu E, Negru A, Docea E. Septoriozele din Romania: Editura Academiei Republicii Socialiste Romania; 1973.
  22. Vanev SG, Sameva EF, Bakalova GG. Order Sphaeropsidales. Fungi Bulgaricae. 1997;3:1-335.