The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Distribution and Species Diversity of Wild Yeasts Isolated from Flowers in Korea

국내에 서식하는 꽃에서 분리한 야생 효모 분포 및 종 다양성

  • Kim, Jeong-Seon (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA) ;
  • Lee, Miran (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA) ;
  • Kim, Jae Yoon (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA) ;
  • Heo, jun (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA) ;
  • Kwon, Soon-Wo (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA) ;
  • Yun, Bong Sik (Division of Biotechnology and Advanced institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kim, Soo-Jin (Agricultural Microbiology Division. National Institute of Agricultural Science, RDA)
  • 김정선 (국립농업과학원 농업미생물과) ;
  • 이미란 (국립농업과학원 농업미생물과) ;
  • 김재윤 (국립농업과학원 농업미생물과) ;
  • 허준 (국립농업과학원 농업미생물과) ;
  • 권순우 (국립농업과학원 농업미생물과) ;
  • 윤봉식 (전북대학교 환경생명자원대학 생명공학부) ;
  • 김수진 (국립농업과학원 농업미생물과)
  • Received : 2020.10.30
  • Accepted : 2020.11.30
  • Published : 2020.12.31
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Abstract

Various indigenous yeasts were isolated and obtained from flowers in the Republic of Korea, and their distribution and species diversity were studied. Seventy-seven flowers were collected from 25 areas in Korea, and 502 yeast strains were isolated from these flowers. A total of 50 species were identified by comparing large subunit rDNA gene sequence homology with the type strains of yeasts. The analysis of yeast distribution showed that the dominant yeast species were Aureobasidium pullulans, A. leucospermi, and Filobasidium magnum in each region and flower samples. Except for the above three yeast species, no species of yeasts showed any meaningful distribution among the habitat regions and sources. In conclusion, 50 species of indigenous yeasts were obtained from flowers that can be used as industrial resources, and the data could be used for further research on yeast diversity and interactions between yeast and its host.

국내에 서식하는 꽃에서 다양한 토착 효모를 분리 및 확보하고 효모의 분포 특성과 종 다양성에 관해 연구하였다. 국내 25지역에서 꽃 9종 77개체를 채집한 후 효모를 순수분리하였다. 효모 502균주의 large-subunit (LSU) rDNA 염기서열을 분석 및 상동성 비교로 각 효모의 종을 동정하였다. 분리된 효모는 총 50종으로 동정 되었고, 모든 꽃 시료로부터 Aureobasidium pullulans, A. leucospermi, Filobasidium magnum이 분리되었다. 꽃에 우점한 효모 3종을 제외하고, 효모 균주 수가 적게 분리된 종은 지역과 분리원에 따른 유의성을 확인할 수는 없었다. 본 연구를 통해 꽃으로부터 확보한 국내 토착 효모 50종은 산업적 활용을 위한 자원으로써 역할을 기대할 수 있고, 추후 효모와 기주 간의 상호작용이나 효모다양성 연구의 기초자료로써 활용될 수 있을 것이다.

Keywords

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