The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes

표고 A 교배형 구분을 위한 CAPS 마커의 개발 및 적용

  • Park, Mi-Jeong (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science) ;
  • Ryoo, Rhim (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science) ;
  • Jang, Yeongseon (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
  • 박미정 (국립산림과학원 산림생명자원연구부 산림소득자원연구과) ;
  • 유림 (국립산림과학원 산림생명자원연구부 산림소득자원연구과) ;
  • 장영선 (국립산림과학원 산림생명자원연구부 산림소득자원연구과) ;
  • 가강현 (국립산림과학원 산림생명자원연구부 산림소득자원연구과)
  • Received : 2020.12.14
  • Accepted : 2020.12.17
  • Published : 2020.12.31
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Abstract

Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other heterothallic basidiomycetes. The A locus of L. edodes encodes a pair of homeodomain (HD) transcription factors. The highly variable N-termini of these HD transcription factors contribute to the diversity among the A mating types. In this study, we developed a cleaved amplified polymorphic sequence (CAPS) marker to discriminate 11 different A mating type alleles predominant among both cultivated and wild strains. Amplification of the variable region of the A locus followed by digestion with HaeIII and EcoRI restriction enzymes enabled successful discrimination among the 11 A mating type alleles. We also evaluated the applicability of this method in the identification of two A mating types of a dikaryotic strain.

표고는 한국에서 가장 많이 소비되는 식용버섯 중 하나이다. 표고는 사극성의 교배계를 따르며, 표고의 교배형은 자웅이주성의 다른 담자균류와 마찬가지로 서로 독립적인 두 유전자좌, A와 B에 의해 결정된다. 표고의 A 유전자좌에는 한 쌍의 homeodomain (HD) 전사인자가 암호화되어 있으며 이들의 N말단에서 나타나는 높은 변이가 A 교배형의 다양성에 중요한 것으로 알려져 있다. 본 연구에서는 표고 품종과 야생종에서 많이 발견되는 11종의 A 교배형을 구분할 수 있는 CAPS 마커를 개발하고자 하였다. A 유전자좌에서 변이가 큰 부분을 PCR을 통해 증폭한 뒤 두 가지 제한효소 HaeIII와 EcoRI로 절단하여 DNA 단편의 크기 및 양상을 살펴봄으로써 11종의 A 교배형을 서로 구별할 수 있었다. 또한 해당 방법이 이핵균주의 교배형을 확인하는 데 활용할 수 있는지도 살펴보았다.

Keywords

Acknowledgement

This work was supported by the Golden Seed Project of 'Breeding of new strains of shiitake for cultivar protection and substitution of import [213007-05-4-SBH10]' provided by the Ministry of Agriculture, Food and Rural Affairs, Ministry of Oceans and Fisheries, Rural Development Administration and Korea Forest Service.

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