Ecology and Resilient Infrastructure

Korean Society of Ecology and Infrastructure Engineering (응용생태공학회)
권호 표지
DOI QR코드

DOI QR Code

Biopolymer Amended Soil Reduces the Damages of Zn Excess in Camlina sativa L.

토양 내 바이오폴리머 혼합에 의한 Camelina sativa L.의 Zn 과잉 스트레스 피해 경감 효과

  • Shin, Jung-Ho (Department of Integrated Food, Bioscience and Biotechnology) ;
  • Kim, Hyun-Sung (Department of Bioenergy Science and Technology, Chonnam National University) ;
  • Kim, Eunsuk (Department of Earth Science and Environmental Engineering, Gwangju Institute of Science and Technology School) ;
  • Ahn, Sung-Ju (Department of Bioenergy Science and Technology, Chonnam National University)
  • 신정호 (전남대학교 융합식품바이오공학과) ;
  • 김현성 (전남대학교 바이오에너지공학과) ;
  • 김은석 (광주과학기술원 지구 환경공학부) ;
  • 안성주 (전남대학교 바이오에너지공학과)
  • Received : 2020.10.27
  • Accepted : 2020.11.10
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Amending biopolymers such as β-glucan (BG) and Xanthan gum (XG) generally enhances soil strength by ionic and hydrogen bonds between soil particles. Thus, biopolymers have been studied as eco-friendly construction materials in levees. However, physiological responses of plants grown on soil amended with biopolymers are not fully understood. This study focuses on the effects of biopolymers on the growth of Camelina sativa L. (Camelina) under excess zinc (Zn) stress. The optimal concentrations of BG and XG were confirmed to have a 0.5% ratio in soil depending on the physiological parameters of Camelina under excess Zn stress. The Zn binding capacity of biopolymers was investigated using 1,5-diphenylthiocarbazone (DTZ). The reduction of Zn damage in Camelina was evaluated by analyzing the Zn content and expression of heavy metal ATPase (HMA) genes under excess Zn stress. Amendments of BG and XG improved Camelina growth under excess Zn stress. In DTZ staining and ICP-OES analysis, Camelina grown on BG and XG soil showed less Zn uptake than normal soil under excess Zn stress. The Zn-inducible CsHMA3 gene was not stimulated by either BG or XG amendment under excess Zn stress. Moreover, both BG and XG amendments in soil exhibit Zn-stress mitigation similar to that of Zn-tolerant CsHMA3 overexpres sed Camelina. These results indicate that biopolymer-amended soils may influence the prevention of Zn absorption in Camelina under excess Zn stress. Thus, BG and XG are proven to be suitable materials for levee construction and can protect plants from soil contamination by Zn.

본 연구에서 사용한 바이오폴리머 (biopolymer)는 친환경 제방 건설 소재로 연구 되고 있다. 이러한 바이오폴리머를 토양에 혼합할 경우 이온결합 및 수소결합을 통해 토양의 강도 증진 효과가 잘 알려져 있지만 바이오폴리머와 혼합된 토양이 식물에 미치는 영향은 자세히 알려져 있지 않다. 본 연구는 Zn 과잉 스트레스 조건에서 바이오폴리머의 토양 혼합이 Camelina sativa L. (Camelina)에 미치는 영향을 분석 하였다. Camelina의 생장실험을 기반으로, Zn 과잉 스트레스에 대한 최적의 바이오폴리머 혼합 비율은 0.5%로 결정하여 연구를 진행하였다. Zn 과잉 스트레스 하에서, BG 또는 XG 혼합구의 Camelina는 바이오폴리머 비혼합구에 비해 높은 생장을 보였으며, Zn 과잉 스트레스 피해 지표인 Malondialdehyde (MDA) 함량과 전해질유출도의 감소를 나타냈다. 바이오폴리머의 Zn 결합능을 DTZ (1,5-diphenylthiocarbazone)을 이용하여 분석한 결과, BG 또는 XG 모두 명확한 Zn 흡착 반응을 보였다. DTZ 염색 및 ICP-OES 분석에서, Zn 과잉 스트레스에 의한 Camelina의 Zn 흡수량이 BG 또는 XG 혼합에 의해 현저히 감소함을 확인하였다. 그리고, BG 또는 XG의 혼합은 Camelina의 중금속 수송체 Heavy metal ATPase (HMA)의 발현을 유도하지 않았으며, 야생형 (wildtype, WT)보다 CsHMA3가 과발현 된 Camelina에서 BG 또는 XG 혼합구와 유사한 수준의 Zn 과잉 스트레스 저감 효과를 확인하였다. 결론적으로, 바이오폴리머의 토양 혼합은 바이오폴리머와 Zn 사이의 결합에 의해 Camelina에 과도한 Zn 이온이 흡수되는 것을 방지하여 Zn 독성 피해를 감소시키는 것으로 확인되었다. 더 나아가 바이오폴리머의 토양혼합은 제방강화뿐만 아니라 중금속으로 오염된 토양에서 식물 생존에 긍정적으로 작용할 것이라 판단된다.

Keywords

References

  1. Al-Wabel, M.I., Usman, A.R., El-Naggar, A.H., Aly, A.A., Ibrahim, H.M., Elmaghraby, S., and Al-Omran, A. 2015. Conocarpus biochar as a soil amendment for reducing heavy metal availability and uptake by maize plants. Saudi Journal of Biological Sciences 22(4): 503-511. https://doi.org/10.1016/j.sjbs.2014.12.003
  2. Arrivault, S., Senger, T., and Kramer, U. 2006. The Arabidopsis metal tolerance protein AtMTP3 maintains metal homeostasis by mediating Zn exclusion from the shoot under Fe deficiency and Zn oversupply. The Plant Journal 46(5): 861-879. https://doi.org/10.1111/j.1365-313X.2006.02746.x
  3. Babcsanyi, I., Tamas, M., Szatmari, J., Hambek-Olah, B., and Farsang, A. 2020. Assessing the impacts of the main river and anthropogenic use on the degree of metal contamination of oxbow lake sediments (Tisza River Valley, Hungary). Journal of Soils and Sediments 20(3): 1662-1675. https://doi.org/10.1007/s11368-019-02516-y
  4. Becher, M., Talke, I.N., Krall, L., and Kramer, U. 2004. Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri. The Plant Journal 37(2): 251-268. https://doi.org/10.1046/j.1365-313X.2003.01959.x
  5. Bergmann, D., Furth, G., and Mayer, C. 2008. Binding of bivalent cations by xanthan in aqueous solution. International Journal of Biological Macromolecules 43(3): 245-251. https://doi.org/10.1016/j.ijbiomac.2008.06.001
  6. Chaney, R.A. 1993. Zinc phytotoxicity. Zinc in soils and plants. Springer. pp. 135-150.
  7. Chang, I. and Cho, G.C. 2012. Strengthening of Korean residual soil with β-1, 3/1, 6-glucan biopolymer. Construction and Building Materials 30: 30-35. https://doi.org/10.1016/j.conbuildmat.2011.11.030
  8. Chang, I., Im, J., Prasidhi, A.K., and Cho, G.C. 2015. Effects of Xanthan gum biopolymer on soil strengthening. Construction and Building Materials 74: 65-72. https://doi.org/10.1016/j.conbuildmat.2014.10.026
  9. Chen, C.L., Cui, Y., Cui, M., Zhou, W.J., Wu, H.L., and Ling, H.Q. 2018. A FIT-binding protein is involved in modulating iron and zinc homeostasis in Arabidopsis. Plant, Cell and Environment 41(7): 1698-1714. https://doi.org/10.1111/pce.13321
  10. Clijsters, H. and Van Assche, F. 1985. Inhibition of photosynthesis by heavy metals. Photosynthesis Research 7(1): 31-40. https://doi.org/10.1007/BF00032920
  11. Courbot, M., Willems, G., Motte, P., Arvidsson, S., Roosens, N., Saumitou-Laprade, P., and Verbruggen, N. 2007. A major quantitative trait locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase. Plant Physiology 144(2): 1052-1065. https://doi.org/10.1104/pp.106.095133
  12. Eren, E. and Arguello, J.M. 2004. Arabidopsis HMA2, a divalent heavy metal-transporting PIB-type ATPase, is involved in cytoplasmic Zn2+ homeostasis. Plant Physiology 136(3): 3712-3723. https://doi.org/10.1104/pp.104.046292
  13. Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., and Maeshima, M. 2011. iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis. Plant Physiology 155(4): 1893-1907. https://doi.org/10.1104/pp.110.169730
  14. Ghorai, S., Sarkar, A.K., and Pal, S. 2014. Rapid adsorptive removal of toxic Pb2+ ion from aqueous solution using recyclable, biodegradable nanocomposite derived from templated partially hydrolyzed xanthan gum and nanosilica. Bioresource Technology 170: 578-582. https://doi.org/10.1016/j.biortech.2014.08.010
  15. Hamidifar, H., Keshavarzi, A., and Truong, P. 2018. Enhancement of river bank shear strength parameters using Vetiver grass root system. Arabian Journal of Geosciences 11(20): 611. https://doi.org/10.1007/s12517-018-3999-z
  16. Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125(1): 189-198. https://doi.org/10.1016/0003-9861(68)90654-1
  17. Jakobik-Kolon, A., Bok-Badura, J., Karon, K., Mitko, K., and Milewski, A. 2017. Hybrid pectin-based biosorbents for zinc ions removal. Carbohydrate polymers 169: 213-219. https://doi.org/10.1016/j.carbpol.2017.03.095
  18. Kim, H.-S., Oh, J.-M., Luan, S., Carlson, J.E., and Ahn, S.-J. 2013. Cold stress causes rapid but differential changes in properties of plasma membrane H+-ATPase of camelina and rapeseed. Journal of Plant Physiology 170: 828-837. https://doi.org/10.1016/j.jplph.2013.01.007
  19. Kim, Y.Y., Choi, H., Segami, S., Cho, H.T., Martinoia, E., Maeshima, M., and Lee, Y. 2009. AtHMA1 contributes to the detoxification of excess Zn (II) in Arabidopsis. The Plant Journal 58(5): 737-753. https://doi.org/10.1111/j.1365-313X.2009.03818.x
  20. Kramer, U. and Clemens, S. 2005. Functions and homeostasis of zinc, copper, and nickel in plants. In Molecular biology of metal homeostasis and detoxification. Springer. pp. 215-271.
  21. Lim, H.G., Kim, H.S., Lee, H.S., Sin, J.H., Kim, E.S., Woo, H.S., and Ahn, S.J. 2018. Amended soil with biopolymer positively affects the growth of Camelina sativa L. under drought stress. Ecology and Resilient Infrastructure 5(3): 163-173. (in Korean) https://doi.org/10.17820/eri.2018.5.3.163
  22. Liu, H., Zhao, H., Wu, L., Liu, A., Zhao, F.J., and Xu, W. 2017. Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola. New Phytologist 215(2): 687-698. https://doi.org/10.1111/nph.14622
  23. Morel, M., Crouzet, J., Gravot, A., Auroy, P., Leonhardt, N., Vavasseur, A., and Richaud, P. 2009. AtHMA3, a P1B-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis. Plant Physiology 149(2): 894-904. https://doi.org/10.1104/pp.108.130294
  24. Palmer, C.M. and Guerinot, M.L. 2009. Facing the challenges of Cu, Fe and Zn homeostasis in plants. Nature Chemical Biology 5(5): 333. https://doi.org/10.1038/nchembio.166
  25. Park, W., Feng, Y., and Ahn, S.J. 2014. Alteration of leaf shape, improved metal tolerance, and productivity of seed by overexpressionn of CsHMA3 in Camelina sativa. Biotechnology for Biofuels 7(1): 96. https://doi.org/10.1186/1754-6834-7-96
  26. Park, W., Feng, Y., Kim, H., Suh, M.C., and Ahn, S.J. 2015. Changes in fatty acid content and composition between wild type and CsHMA3 overexpressing Camelina sativa under heavy-metal stress. Plant Cell Reports 34(9): 1489-1498. https://doi.org/10.1007/s00299-015-1801-1
  27. Puga, A.P., Abreu, C.A., Melo, L.C.A., and Beesley, L. 2015. Biochar application to a contaminated soil reduces the availability and plant uptake of zinc, lead and cadmium. Journal of Environmental Management 159: 86-93. https://doi.org/10.1016/j.jenvman.2015.05.036
  28. Seigneurin-Berny, D., Gravot, A., Auroy, P., Mazard, C., Kraut, A., Finazzi, G., Grunwald, D., Rappaport, F., Vavasseur, A., and Joyard, J. 2006. HMA1, a new Cu-ATPase of the chloroplast envelope, is essential for growth under adverse light conditions. Journal of Biological Chemistry 281(5): 2882-2892. https://doi.org/10.1074/jbc.M508333200
  29. Shanmugam, V., Lo, J.C., Wu, C.L., Wang, S.L., Lai, C.C., Connolly, E.L., Huang, J.L., and Yeh, K.C. 2011. Differential expression and regulation of iron-regulated metal transporters in Arabidopsis halleri and Arabidopsis thaliana-the role in zinc tolerance. New Phytologist 190(1): 125-137. https://doi.org/10.1111/j.1469-8137.2010.03606.x
  30. Takahashi, R., Bashir, K., Ishimaru, Y., Nishizawa, N.K., and Nakanishi, H. 2012. The role of heavy-metal ATPases, HMAs, in zinc and cadmium transport in rice. Plant Signaling and Behavior 7(12): 1605-1607. https://doi.org/10.4161/psb.22454
  31. Vahedifard, F., AghaKouchak, A., and Robinson, J.D. 2015. Drought threatens California's levees. Science 349(6250): 799.
  32. Vassilev, A., Nikolova, A., Koleva, L., and Lidon, F. 2011. Effects of excess Zn on growth and photosynthetic performance of young bean plants. Journal of Phytology 3(6): 58-62.
  33. Verret, F., Gravot, A., Auroy, P., Leonhardt, N., David, P., Nussaume, L., Vavasseur, A., and Richaud, P. 2004. Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance. FEBS Letters 576(3): 306-312. https://doi.org/10.1016/j.febslet.2004.09.023
  34. Wong, C.K.E. and Cobbett, C.S. 2009. HMA P-type ATPases are the major mechanism for root-to-shoot Cd translocation in Arabidopsis thaliana. New Phytologist 181(1): 71-78. https://doi.org/10.1111/j.1469-8137.2008.02638.x