한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Polysiponia morrowii Extract Inhibits Cancer Growth on CT-26 and Hela cells

  • Zhang, Chunying (Department of Integrated of Bioindustry, Hanseo University) ;
  • Cha, Seon-Heui (Department of Integrated of Bioindustry, Hanseo University)
  • 투고 : 2020.11.24
  • 심사 : 2020.12.24
  • 발행 : 2020.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cancer is an unfavorable human disease, and the treatment commonly have side effects and can be ineffective. Since exploration and development of cancer treatment drugs is particularly demanding, this study aimed to investigate the anticancer activities of Polysiponia morrowii extract s (PME) on CT-26 and HeLa cells. The results showed that PME inhibited cell proliferation in a dose-dependent manner, with IC50 values of 41.04% in CT-26 and 48.51% in HeLa cell cultures. Moreover, cytological observation using Hoechst 33342 staining assay showed typical apoptotic morphology in both cancer cells, and production of sub-G1 DNA was induced by PME treatment in a dose-dependent manner, with 34.41% in CT-26 and 46.01% in HeLa cell cultures. These findings suggest that PME may have potential preventive effects or medicinal value in the treatment of colorectal and cervical cancers.

키워드

참고문헌

  1. Bray, F., et al., Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 2018. 68(6): p. 394-424. https://doi.org/10.3322/caac.21492
  2. Jung, K.W., et al., Cancer Statistics in Korea: Incidence, Mortality, Survival, and Prevalence in 2015. Cancer Res Treat, 2018. 50(2): p. 303-316. https://doi.org/10.4143/crt.2018.143
  3. Jung, K.W., et al., Prediction of Cancer Incidence and Mortality in Korea, 2019. Cancer Res Treat, 2019. 51(2): p. 431-437. https://doi.org/10.4143/crt.2019.139
  4. Arbyn, M., et al., Estimates of incidence and mortality of cervical cancer in 2018: a world wide analysis. The Lancet Global Health, 2020. 8(2): p. e191-e203. https://doi.org/10.1016/s2214-109x(19)30482-6
  5. Monk, B.J., et al., Phase III trial of four cisplatin-containing doublet combinations in stage IVB, recurrent, or persistent cervical carcinoma: a Gynecologic Oncology Group study. J Clin Oncol, 2009. 27(28): p. 4649-55. https://doi.org/10.1200/jco.2009.21.8909
  6. Johnson, C.A., et al., Cervical Cancer: An Overview of Pathophysiology and Management. Semin Oncol Nurs, 2019. 35(2): p. 166-174. https://doi.org/10.1016/j.soncn.2019.02.003
  7. Fridlender, M., Y. Kapulnik, and H. Koltai, Plant derived substances with anti-cancer activity: from folklore to practice. Front Plant Sci, 2015. 6: p. 799. https://doi.org/10.3389/fpls.2015.00799
  8. Yuan, H., et al., The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 2016. 21(5).
  9. Dyshlovoy, S.A. and F. Honecker, Marine Compounds and Cancer: 2017 Updates. Mar Drugs, 2018. 16(2).
  10. Khalifa, S.A.M., et al., Marine Natural Products: A Source of Novel Anticancer Drugs. Mar Drugs, 2019. 17(9).
  11. Courdavault, V., et al., Towards the Microbial Production of Plant-Derived Anticancer Drugs. Trends Cancer, 2020. 6(6): p. 444-448. https://doi.org/10.1016/j.trecan.2020.02.004
  12. Lukasiewicz, K. and M. Fol, Microorganisms in the Treatment of Cancer: Advantages and Limitations. J Immunol Res, 2018. 2018: p. 2397808.
  13. Lowenthal, R.M. and J.H. Fitton, Are seaweed-derived fucoidans possible future anti-cancer agents? Journal of Applied Phycology, 2014. 27(5): p. 2075-2077. https://doi.org/10.1007/s10811-014-0444-x
  14. Wali, A.F., et al., Natural products against cancer: Review on phytochemicals from marine sources in preventing cancer. Saudi Pharm J, 2019. 27(6): p. 767-777. https://doi.org/10.1016/j.jsps.2019.04.013
  15. Wang, Y., et al., Simulated digestion and fermentation in vitro with human gut microbiota of polysaccharides from Coralline pilulifera. Lwt, 2019. 100: p. 167-174. https://doi.org/10.1016/j.lwt.2018.10.028
  16. Sithranga Boopathy, N. and K. Kathiresan, An ticancer drugs from marine flora: an overview. J Oncol, 2010. 2010: p. 214186.
  17. Alghazeer, R., et al., Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Natural Science, 2018. 10(07): p. 232-246. https://doi.org/10.4236/ns.2018.107025
  18. Yoshie, Y., et al., Compositional Difference of Phenolic Compounds between Two Seaweeds, Halimeda. Journal of Tokyo University of Fisheries, 2002. 88: p. 21-24.
  19. Vasanthi, H.R., et al., Tumoricidal effect of the red algae Acanthophora spicifera on Ehrlich's ascites carcinoma in mice. Seaweed Research and Utilization, 2004. 25: p. 217-224.
  20. Yuan, Y. V., et al., Extracts from dulse (Palmaria palmata) are effective antioxidants and inhibitors of cell proliferation in vitro. Food Chem Toxicol, 2005. 43(7): p. 1073-1081. https://doi.org/10.1016/j.fct.2005.02.012
  21. Lijun, H., et al., Isolation and pharmacological activities of bromophenols from Rhodomela confervoides. Chinese Journal of Oceanology and Limnology, 2005. 23(2): p. 226-229. https://doi.org/10.1007/BF02894243
  22. Geoffroy, A., et al., Patterns of genetic diversity of the cryptogenic red alga Polysiphonia morrowii (Ceramiales, Rhodophyta) suggest multiple origins of the Atlantic populations. Ecol Evol, 2016. 6(16): p. 5635-47. https://doi.org/10.1002/ece3.2135
  23. Pineiro-Corbeira, C., H. Verbruggen, and P. Diaz-Tapia, Molecular survey of the red algal family Rhodomelaceae (Ceramiales, Rhodophyta) in Australia reveals new introduced species. Journal of Applied Phycology, 2019. 32(4): p. 2535-2547. https://doi.org/10.1007/s10811-019-01932-4
  24. D'Archino, R., K.F. Neill, and W.A. Nelson, Recognition and distribution of Polysiphonia morrowii (Rhodomelaceae, Rhodophyta) in New Zealand. Botanica Marina, 2013. 56(1).
  25. Kim, S.Y., et al., In vitro antiviral activity of red alga, Polysiphonia morrowii extract and its bromophenols against fish pathogenic infe ctious hematopoietic necrosis virus and infect ious pancreatic necrosis virus. J Microbiol, 2011. 49(1): p. 102-6. https://doi.org/10.1007/s12275-011-1035-z
  26. Radonic, A., et al., Anionic Polysaccharides From Phototrophic Microorganisms Exhibit Antiviral Activities to Vaccinia Virus. Journal of Antivirals & Antiretrovirals, 2010. 02(04).
  27. Tannin-Spitz, T., et al., Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. Journal of Applied Phycology, 2005. 17(3): p. 215-222. https://doi.org/10.1007/s10811-005-0679-7
  28. Matsui, M.S., et al., Sulfated Polysaccharides from Red Microalgae Have Antiinflammatory Properties In Vitro and In Vivo. Applied Biochemistry and Biotechnology, 2003. 104(1): p. 13-22. https://doi.org/10.1385/ABAB:104:1:13
  29. Jung, W.K., et al., A novel anticoagulant protein from Scapharca broughtonii. J Biochem Mol Biol, 2002. 35(2): p. 199-205.
  30. Gerwick, W.H. and B.S. Moore, Lessons from the past and charting the future of marine natural products drug discovery and chemical biology. Chem Biol, 2012. 19(1): p. 85-98. https://doi.org/10.1016/j.chembiol.2011.12.014
  31. El Gamal, A.A., Biological importance of mar ine algae. Saudi Pharm J, 2010. 18(1): p. 1-25. https://doi.org/10.1016/j.jsps.2009.12.001
  32. Yuan, Y.V. and N.A. Walsh, Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol, 2006. 44(7): p. 1144-50. https://doi.org/10.1016/j.fct.2006.02.002
  33. Paul, S. and R. Kundu, Antiproliferative activity of methanolic extracts from two green algae, Enteromorpha intestinalis and Rizoclonium riparium on HeLa cells. Daru, 2013. 21(1): p. 72. https://doi.org/10.1186/2008-2231-21-72
  34. Murugan, K. and V.V. Iyer, Antioxidant and Antiproliferative Activities of Extracts of Selected Red and Brown Seaweeds from the Mandapam Coast of Tamil Nadu. Journal of Food Biochemistry, 2014. 38(1): p. 92-101. https://doi.org/10.1111/jfbc.12029
  35. Kang, S., et al., In vitro antiviral activities of Korean marine algae extracts against fish pathogenic infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus. Food Science and Biotechnology, 2008. 17: p. 1074-1078.
  36. Choi, Y. K., et al., Bis (3-bromo-4,5-dihydroxy benzyl) ether, a novel bromophenol from the marine red alga Polysiphonia morrowii that suppresses LPS-induced inflammatory response by inhibiting ROS-mediated ERK signaling pathway in RAW 264.7 macrophages. Biomed Pharmacother, 2018. 103: p. 1170-1177. https://doi.org/10.1016/j.biopha.2018.04.121
  37. Zhao, M., et.al., Immunomodulatory and anticancer activities of flavonoids extracted from litchi (Litchi chinensis Sonn.) pericarp. International Immunopharmacology, 2007. 7(2): p. 162-166. https://doi.org/10.1016/j.intimp.2006.09.003
  38. Gawron, A. and I. Kruk, Cytotoxic effect of xanthotoxol (8-hydroxypsoralen) on TCTC cells in vitro. Polish journal of pharmacology and pharmacy, 1992. 44 1: p. 51-7.
  39. Zhao, M., et al., Immunomodulatory and anticancer activities of flavonoids extracted from litchi (Litchi chinensis Sonn.) pericarp. International Immunopharmacology, 2007. 7(2): p. 162-166. https://doi.org/10.1016/j.intimp.2006.09.003
  40. Tao, M.Z., et al., Effects of TGF-beta1 on the Proliferation and Apoptosis of Human Cervical Cancer Hela Cells In Vitro. Cell Biochem Biophys, 2015. 73(3): p. 737-41. https://doi.org/10.1007/s12013-015-0673-x
  41. Syed Abdul Rahman, S.N., N. Abdul Wahab, and S.N. Abd Malek, In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria. Evid Based Complement Alternat Med, 2013. 2013: p. 257108.
  42. Gwak, J., et al., Polysiphonia japonica extract suppresses the Wnt/beta-catenin pathway in colon cancer cells by activation of NF-kappaB. Int J Mol Med, 2006. 17(6): p. 1005-10.