Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of glucosinolates and their metabolites from napa cabbage (Brassica rapa subsp. Pekinensis) and napa cabbage kimchi using UPLC-MS/MS

UPLC-MS/MS를 이용한 배추와 배추김치의 글루코시놀레이트 및 대사체 분석

  • Kim, Jaecheol (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Park, Hyo Sun (Department of Food and Nutrition, Chung-Ang University) ;
  • Hwang, Keum Taek (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Moon, BoKyung (Department of Food and Nutrition, Chung-Ang University) ;
  • Kim, Suna (Division of Human Ecology, College of Natural Science, Korea National Open University)
  • 김재철 (서울대학교 생활과학대학 식품영양학과.생활과학연구소) ;
  • 박효순 (중앙대학교 생명공학대학 식품공학부 식품영양전공) ;
  • 황금택 (서울대학교 생활과학대학 식품영양학과.생활과학연구소) ;
  • 문보경 (중앙대학교 생명공학대학 식품공학부 식품영양전공) ;
  • 김선아 (한국방송통신대학교 자연과학대학 생활과학부 식품영양학전공)
  • Received : 2020.09.23
  • Accepted : 2020.10.22
  • Published : 2020.12.31
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Abstract

In this study, we analyzed glucosinolates and their metabolites in the inner and outer parts of napa cabbage (NC; Brassica rapa subsp. pekinensis) and napa cabbage kimchi (NKC) using UPLC-ESI-MS/MS. In the extracts from NC and NKC, glucobrassicanapin (m/z 386), glucoalyssin (m/z 450), glucobrassicin (m/z 447), 4-methoxyglucobrassicin (m/z 477), and neoglucobrassicin (m/z 477) were detected using the MS scan mode ([M-H]-), and gluconapin (m/z 372→97), progoitrin (m/z 388→97), glucoiberin (m/z 422→97), 4-methoxyglucobrassicin (m/z 477→97), and neoglucobrassicin (m/z 477→447) were detected using the MS/MS MRM mode ([M-H]-). Ascorbigen (m/z 306→130) and indole-3-carboxaldehyde (I3A; m/z 146→118), which were metabolites of glucobrassicins, were detected using the MS/MS MRM ([M+H]+) mode. The peak intensities of ascorbigen in the extract from the inner and outer parts of NC were significantly higher than those of the NKC extract (p<0.05); however, there was no significant difference in I3A peak intensity between the NC and NKC extracts.

본 연구에서는 부위별 배추와 배추김치에 함유되어 있는 글루코시놀레이트류의 조성을 UPLC-MS/MS를 이용하여 탐색하고, 인돌류 대사체를 MS/MS를 이용하여 분석한 결과, 글루코시놀레이트류는 음이온 모드([M-H]-)에서 검출되었으며, glucobrassicanapin (m/z 386), glucoalyssin (m/z 450), glucobrassicin (m/z 447), 4-methoxyglucobrassicin (m/z 477), neoglucobrassicin (m/z 477)는 MS scan 모드에서, gluconapin (m/z 372→97), progoitrin (m/z 388→97), glucoiberin (m/z 422→97), 4-methoxyglucobrassicin (m/z 477→97), neoglucobrassicin (m/z 477→447)는 MS/MS MRM 모드에서 검출되었다. Glucobrassicin과 같은 인돌기 함유 글루코시놀레이트류들이 대사되어 생성되는 인돌류 대사체로는 ascorbigen (m/z 306→130)과 I3A (m/z 146→118)가 MS/MS MRM ([M+H]+) 모드에서 검출되었다. Ascorbigen은 NKC보다 IPC와 OPC에 유의적으로 많이 함유되어 있었다. I3A는 NKC에 가장 많이 함유되어 있었으나 시료 간 유의적인 차이는 없었다. 본 연구를 통하여 배추와 배추김치에 존재하는 글루코시놀레이트류와 그 대사체인 인돌류 물질을 UPLC-MS/MS를 이용하여 분석할 수 있었다.

Keywords

References

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