Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Variey Discrimination of Sorghum-Sudangrass Hybrids Seed Using near Infrared Spectroscopy

근적외선분광법을 이용한 수수×수단그라스 교잡종 종자의 품종 판별

  • Lee, Ki-Won (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Song, Yowook (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ji Hye (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Rahman, Md Atikur (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Mirae (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2020.11.16
  • Accepted : 2020.11.24
  • Published : 2020.12.31
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Abstract

The aim of this study was to investigate the feasibility of discrimination 12 different cultivar of sorghum × sudangrass hybrid (Sorghum genus) seed through near infrared spectroscopy (NIRS). The amount of samples for develop to the best discriminant equation was 360. Whole samples were applied different three spectra range (visible, NIR and full range) within 680-2500 nm wavelength and the spectrastar 2500 Near near infrared was used to measure spectra. The calibration equation for discriminant analysis was developed partial least square (PLS) regression and discrimination equation (DE) analysis. The PLS discriminant analysis model for three spectra range developed with mathematic pretreatment 1,8,8,1 successfully discriminated 12 different sorghum genus. External validation indicated that all samples were discriminated correctly. The whole discriminant accuracy shown 82 ~ 100 % in NIR full range spectra. The results demonstrated the usefulness of NIRS combined with chemometrics as a rapid method for discrimination of sorghum × sudangrass hybrid cultivar through seed.

본 연구는 근적외선분광법을 이용하여 국내에서 재배중인 수수×수단그라스 교잡종 품 판별 가능성을 검토하고자 수행되었다. 근적외선분광기를 이용하여 수수×수단그라스 교잡종 종자를 가시파장 대역대 (680 - 1,099 nm), NIRS 파장 대역대 (1,100 - 2,500 nm) 및 NIRS 전체 파장 대역대 (680 - 2,500 nm)로 구분하여 스펙트라를 얻은 후 1차 미분과 8 nm gap으로 수 처리를 수행하였으며 부분최소자승 (PLS) 회귀분석법을 통해 품종판별 검량식을 개발하고 판별 정확성을 검증하였다. 수수×수단그라스 교잡종품종 판별의 정확성은 NIR파장대역에서 SECV 8.44 그리고 R2CV 0.89로 가장 판별 정확성이 낮았으며 NIRS 전체 파장대역에서 SECV 7.88 그리고 R2CV 0.90로 가장 높은 판별 정확성을 나타내었다. 파장대역별 예측 정확성은 NIR 파장대역 (1,100 - 2,500 nm)이 가장 우수하였으며, 교차검증오차 (SECV) 8.44에서 예측오차 (SEP) 12.03로 높아졌으며 가시영역대 (680 - 1,099)는 SECV 8.23에서 SEP 12.51로 높아졌다. Discrimination equation 분석법에 의한 NIRS 전체 파장대역별 수수×수단그라스 교잡종 종자의 판별 결과는 품종간에 판별 정확성의 차이가 크게 나타났으며 1, 2, 4 그리고 8번 품종 (G-7, BMR Gold II, Honey chew and SX-17)에서는 100 %의 정확성으로 가장 높게 나타났다. 따라서 NIRS를 이용한 수수×수단그라스 교잡종 종자의 판별분석이 가능할 것으로 판단되었다.

Keywords

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