Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Dermal Papilla Cells Proliferation Constituent of Schisandra chinensis Fruits and Optimization Using Response Surface Methodology

오미자의 모유두세포 증식 활성성분과 반응표면분석을 이용한 추출조건의 최적화

  • Cho, Hyun Dae (College of Pharmacy, Chungbuk National University) ;
  • Jeong, JiYeon (Bio Convergence R&D Center, CoSeedBioPharm Corporation) ;
  • Ryu, Hwa Sun (Bio Convergence R&D Center, CoSeedBioPharm Corporation) ;
  • Lee, JungNo (Bio Convergence R&D Center, CoSeedBioPharm Corporation) ;
  • Park, Sung-Min (Bio Convergence R&D Center, CoSeedBioPharm Corporation)
  • 조현대 (충북대학교 약학대학) ;
  • 정지연 ((주)코씨드바이오팜 바이오융합연구소) ;
  • 류화선 ((주)코씨드바이오팜 바이오융합연구소) ;
  • 이정노 ((주)코씨드바이오팜 바이오융합연구소) ;
  • 박성민 ((주)코씨드바이오팜 바이오융합연구소)
  • Received : 2020.10.12
  • Accepted : 2020.12.23
  • Published : 2020.12.30
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Abstract

In the present study, we have refined gomisin N, which represents activity in the proliferation of dermal papilla cells (HFDPCs) from the fruit of Schisandra chinensis (S. chinensis), and have identified optimal extraction conditions for obtaining extracts with high content of gomisin N. The activity of the extracts and fractions was evaluated, and the results indicated approximately 29% proliferation activity in the group treated with 1 ㎍/mL of n-hexane fraction. Column chromatography was used to assess the active ingredient in the n-hexane fraction, and two compounds, namely gomisin N(1) and schisandrin(2), were isolated and identified. When the HFDPCs proliferation activity was tested for the isolated compounds, gomisin N exhibited ≥ 20% proliferation activity. Thus, via response surface methodology (RSM), the optimum extraction conditions to obtain the maximum level of gomisin N from the fruit of S. chinensis were determined, where ethanol proportion, extraction time, and extraction temperature were used as the independent variables. The results revealed coefficient of determination ≥ 0.95 and p-value ≤ 0.05, which confirmed the fit of the model. The optimum extraction conditions to achieve the maximum content of gomisin N were as follows: ethanol proportion 83.8%, extraction temperature 80 ℃, and extraction time 8.7 h. The content of gomisin N using these conditions was predicted as 378,300 ppm, and a mean value close to the predicted value (376,884 ppm) was obtained while validating the aforementioned conditions.

본 연구에서는 오미자 열매로부터 모유두세포 증식에 활성을 나타내는 gomisin N을 정제하고 gomisin N의 함량이 높은 추출물을 얻기 위한 최적의 추출조건을 확인하였다. 추출물 및 분획물에 대하여 모유두세포 증식 활성 실험을 진행한 결과, n-hexane 분획물 1 ㎍/mL 처리군에서 약 29%의 증식 활성을 나타내었다. n-Hexane 분획물의 활성성분을 규명하기 위하여 column chromatography를 진행하였으며 2 개의 화합물 gomisin N (1)과 schizandrin (2)를 분리 동정하였다. 분리된 화합물에 대한 모유두세포 증식 활성 실험 결과, gomisin N에서 최대 20%이상의 증식 활성을 나타내었다. 따라서 오미자 열매로부터 최대치의 gomisin N을 얻을 수 있는 최적추출조건을 반응표면분석(RSM)을 사용하여 확인하였다. 최적추출조건을 설정하기 위해 에탄올의 비율, 추출시간, 추출온도를 독립변수로 하여 실험한 결과, 0.95 이상의 결정계수와 0.05 이하의 p-value를 나타내어 모델의 적합성이 확인 되었다. Gomisin N의 최대함량을 위한 최적추출조건은 에탄올 비율 83.8%, 추출온도 80 ℃, 추출시간 8.7 h으로 확인되었고, 최적추출조건에서 gomisin N의 함량은 378,300 ppm으로 예측되었다. 최적추출조건에 대한 실제 검증에서 평균 376,884 ppm으로 예측된 값과 유사한 결과를 확인 할 수 있었다

Keywords

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