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The Korean Society of Phycology (한국조류학회(藻類))
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Integrative taxonomic description of two new species of the Cocconeis placentula group (Bacillariophyceae) from Korea based on unialgal strains

  • Jahn, Regine (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin) ;
  • Abarca, Nelida (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin) ;
  • Kusber, Wolf-Henning (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin) ;
  • Skibbe, Oliver (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin) ;
  • Zimmermann, Jonas (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin) ;
  • Mora, Demetrio (Botanischer Garten und Botanisches Museum Berlin, Freie Universitat Berlin)
  • Received : 2020.06.02
  • Accepted : 2020.08.01
  • Published : 2020.12.15
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Abstract

Cocconeis coreana and C. sijunghoensis are described as new based on micromorphological and molecular data. C. coreana is represented by five unialgal cultures from four different freshwater bodies, two from North Korea and three from South Korea. C. sijunghoensis is represented by two unialgal cultures from a brackish water body in North Korea. Except for one, all of the strains auxosporulated and showed an almost quadrupling of size in length and width. Morphologically, these species with their two different elliptical valves belong to the Cocconeis placentula group. The raphe valve has striae with uniseriate areolae continuing across a pronounced submarginal hyaline rim to the edge of the valve. The sternum valve has uniseriate dash-like areolae continuously from the valve face until the valve edge. Micromorphologically, these species possess two different open valvocopulae: only the raphe valvocopula has fimbriae; the sternum valvocopula has none. Based on p-distances of currently available DNA sequence data, i.e., rbcL and 18SV4, both species are pronouncedly different from the epitype strain of C. placentula, with C. coreana closest to the published molecular data of the strain UTEX FD23 named C. placentula from Iowa, USA, while C. sijunghoensis is closest but not the same as the published molecular data of strain D36_012, the epitype strain of C. placentula from Berlin, Germany. Based on scanning electron microscope observations, differentiating features are discussed concerning valvocopula fimbriae, central area, areolation of the sternum valve and on the raphe valve especially between the submarginal hyaline rim and edge.

Keywords

Acknowledgement

The authors are grateful to Dr. Suh Byung Moon for his contribution to the description of these new species and for his longtime support in collecting diatoms from Korea. They also thank Jana Bansemer for work in the molecular lab, Juliane Bettig and Monika Lüchow at the SEM, and Michael Rodewald for help with the plates. The authors acknowledge funding by the Friends of the BGBM for cultivation support, the Deutsche Forschungsgemeinschaft for SEM [INST130/839-1FUGG], for Names Registration [JA874/8-1], and by the Bundesministerium für Bildung und Forschung [01LI1501E] for GBOL2 Diatoms, and by the Swedish Research Council for Sustainable Development (FORMAS) for cooperation.

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