Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Higher Production of Biolipids from Botryococcus braunii using Pre-treated Solvent Extraction Methods

해양생물 Botryococcus braunii에서 유래한 바이오연료의 고급생산기술: 전처리 용매추출법

  • Kwon, Sung-Hyun (Department of Marine Environmental Engineering, Gyeongsang National University) ;
  • Cho, Daechul (Department of Energy & Environmental Engineering, Soonchunhyang University)
  • 권성현 (경상대학교 해양과학대학 해양환경공학과) ;
  • 조대철 (순천향대학교 에너지환경공학과)
  • Received : 2019.07.22
  • Accepted : 2019.11.04
  • Published : 2019.11.30
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Abstract

A lipid-enriched strain of Botryococcus braunii (UTEX 572) was cultivated in a semi-batch aeration tank to enhance biomass as well as to develop intracellular lipids and fatty acids. A 30 day period of incubation produced 1.39 g/L of biomass and 0.31 g/L of total lipids in the biomass. The grown biomass was pre-treated using several methods to extract the total lipid content efficiently: ultrasonication was found to yield the highest percentage of lipids-namely 19.8% per biomass. Direct heating of biomass in an autoclave also showed better performance than when using only conventional solvent extraction. To enhance the biomass harvest and lipid extraction efficiency, coagulation and flocculation steps were added to the extraction process. It is noteworthy that not only the solvent type but also the solvent/biomass ratio greatly affected efficiency. In addition, the moisture content of the harvested(wet) biomass affected the efficiency significantly. This study elucidated the need for future research on optimizing this extraction process.

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References

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