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Study on the Cell Adhesion of Breast Cancer Cells using Nano/Micro Patterning PDMS

나노/마이크로 패턴 PDMS를 이용한 유방암 세포의 부착에 관한 연구

  • Kwak, Do Hoon (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Kim, Woo Cheol (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Jin, Hee Won (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Yun, Wan Su (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Park, Sanghyo (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Key, Jaehong (Department of Biomedical Engineering, College of Health Sciences, Yonsei University)
  • 곽도훈 (연세대학교 보건과학대학 의공학부) ;
  • 김우철 (연세대학교 보건과학대학 의공학부) ;
  • 진희원 (연세대학교 보건과학대학 의공학부) ;
  • 윤완수 (연세대학교 보건과학대학 의공학부) ;
  • 박상효 (연세대학교 보건과학대학 의공학부) ;
  • 기재홍 (연세대학교 보건과학대학 의공학부)
  • Received : 2019.05.21
  • Accepted : 2019.10.04
  • Published : 2019.10.31

Abstract

Cancer cells are different from normal cells in terms of life cycle, behavior, and growth patterns. Cancer cells can migrate freely in the body through blood vessels and lymph nodes. The cancer cells easily interact with various substrates including extracellular matrix and vessels and they can differentiate in the new environment. However, it is not well known about the adhesion preference of cancer cells on the substrate and the mechanism of their interaction. In this study, we prepared the nano-, micro-patterned substrates using E-beam lithography techniques. MCF-7 cells were tested on the substrates to find out their adhesion preference. The substrates were made by polydimethylsiloxane (PDMS) with specific patterns including pillars with a diameter of 500 nm, 700 nm, $3{\mu}m$ and $5{\mu}m$. MCF-7 cells were seeded on the substrates and incubated for 24 hours. As a result, this study clearly demonstrated that the MCF-7 cells preferred 700 nm patterning.

Keywords

References

  1. Boyan BD, Hummert TW, Dean DD, Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials. 1996;17:137-46. https://doi.org/10.1016/0142-9612(96)85758-9
  2. Cukierman E, Pankov R, Stevens DR, and Yamada KM. Taking cell-matrix adhesions to the third dimension. Science. 2001;294:1708-12. https://doi.org/10.1126/science.1064829
  3. Grayson WL, Ma T, Bunnell B. Human mesenchymal stem cells tissue development in 3D PET matrices. Biotechnol Prog. 2004;20:905-12. https://doi.org/10.1021/bp034296z
  4. Lim JY, Donahue HJ. Biomaterial characteristics important to skeletal tissue engineering. J Musculoskelet Neuronal Interact. 2004;4:396-8.
  5. Kostic A, Lynch, CD, Sheetz MP. Differential matrix rigidity response in breast cancer cell lines correlates with the tissue tropism. PLoS One. 2009;4:e6361. https://doi.org/10.1371/journal.pone.0006361
  6. Tilghman RW, Cowan CR, Mih JD, Koryakina Y, Gioeli D, Slack-Davis JK, et al. Matrix rigidity regulates cancer cell growth and cellular phenotype. PLoS One. 2010;5:e12905. https://doi.org/10.1371/journal.pone.0012905
  7. Tzvetkova-Chevolleau T, Stephanou A, Fuard D, Ohayon J, Schiavone P, Tracqui P. The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure. Biomaterials. 2008;29:1541-51. https://doi.org/10.1016/j.biomaterials.2007.12.016
  8. De Silva MN, Desai R, Odde DJ. Micro-patterning of animal cells on PDMS substrates in the presence of serum without use of adhesion inhibitors. Biomedical microdevices. 2004;6:219-22. https://doi.org/10.1023/B:BMMD.0000042051.09807.8c
  9. Lim JY, Donahue HJ. Cell sensing and response to microand nanostructured surfaces produced by chemical and topographic patterning. Tissue Eng. 2007;13:1879-91. https://doi.org/10.1089/ten.2006.0154
  10. Bae J, Oh E, Lee H, Key J. Improved Manufacturing Method of Discoidal Nanoparticles for Cancer Theranostics. Journal of Biomedical Engineering Research. 2016;37:46-52. https://doi.org/10.9718/JBER.2016.37.1.46
  11. Fuard D, Tzvetkova-Chevolleau T, Decossas S, Tracqui P, Schiavone P. Optimization of poly-di-methyl-siloxane (PDMS) substrates for studying cellular adhesion and motility. Microelectronic Engineering. 2008;85:1289-93. https://doi.org/10.1016/j.mee.2008.02.004
  12. Palchesko RN, Zhang L, Sun Y, Feinberg AW. Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve. PLoS One. 2012;7:e51499. https://doi.org/10.1371/journal.pone.0051499
  13. Mata A, Boehm C, Fleischman AJ, Muschler G, Roy S. Growth of connective tissue progenitor cells on microtextured polydimethylsiloxane surfaces. J Biomed Mater Res. 2002;62:499-506. https://doi.org/10.1002/jbm.10353
  14. Fletcher DA, Mullins RD. Cell mechanics and the cytoskeleton. Nature. 2010;463:485-92. https://doi.org/10.1038/nature08908
  15. Geli MI, Riezman H. Endocytic internalization in yeast and animal cells: similar and different. J Cell Sci. 1998;111:1031-7. https://doi.org/10.1242/jcs.111.8.1031
  16. Harries PA, Schoelz JE, Nelson RS. Intracellular transport of viruses and their components: utilizing the cytoskeleton and membrane highways. Mol Plant Microbe Interact. 2010;23:1381-93. https://doi.org/10.1094/MPMI-05-10-0121
  17. Discher DE, Janmey P, Wang YL. Tissue cells feel and respond to the stiffness of their substrate. Science 2005;310:1139-43. https://doi.org/10.1126/science.1116995
  18. Curtis A, Riehle, M. Tissue engineering: the biophysical background. Phys Med Biol. 2001;46:R47-65. https://doi.org/10.1088/0031-9155/46/4/201