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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Multiplexed single-molecule flow-stretching bead assay for DNA enzymology

  • Lee, Ryanggeun (Department of Physics, Pohang University of Science and Technology (POSTECH)) ;
  • Yang, Keunsang (School of Interdisciplinary Bioscience and Bioengineering, POSTECH) ;
  • Lee, Jong-Bong (Department of Physics, Pohang University of Science and Technology (POSTECH))
  • Received : 2019.07.09
  • Published : 2019.10.31
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Abstract

Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high spatiotemporal resolutions of ~nanometers and ~milliseconds. However, DNA metabolism results from coordination of a number of components during the processes, requiring efficient monitoring of a complex of proteins catalyzing DNA substrates. In this min-review, we will introduce a simple and multiplexed single-molecule assay to detect DNA substrates catalyzed by enzymes with high-throughput data collection. We conclude with a perspective of possible directions that enhance capability of the assay to reveal complex biological events with higher resolution.

Keywords

References

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