Mycobiology

The Korean Society of Mycology (한국균학회)
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Bio-Sulfur Pre-Treatment Suppresses Anthracnose on Cucumber Leaves Inoculated with Colletotrichum orbiculare

  • Ko, Eun Ju (College of Applied Life Science, Major of Plant Resources and Environment, Jeju National University) ;
  • Shin, Yong Ho (College of Applied Life Science, Major of Plant Resources and Environment, Jeju National University) ;
  • Hyun, He Nam (College of Applied Life Science, Major of Plant Resources and Environment, Jeju National University) ;
  • Song, Hyo Soon (Eco Energy Holdings Co., Ltd) ;
  • Hong, Jeum Kyu (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Jeun, Yong Chull (College of Applied Life Science, Major of Plant Resources and Environment, Jeju National University)
  • Received : 2018.10.16
  • Accepted : 2019.05.17
  • Published : 2019.09.30
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Abstract

Bio-sulfur can be produced in the process of desulfurization from a landfill and collected by some microorganism such as Thiobacillus sp. as a sulfur element. In order to investigate practical use of bio-sulfur as an agent for controlling plant disease, in vitro antifungal activity of bio-sulfur was tested against Colletotrichum orbiculare known to cause cucumber anthracnose. Efficacy of bio-sulfur for suppressing anthracnose disease was also evaluated in vivo using cucumber leaves. Mycelial growth of C. orbiculare on medium containing bio-sulfur was inhibited. Disease severity of cucumber leaves pre-treated with bio-sulfur was significantly decreased compared to that of untreated ones. To illustrate how bio-sulfur could suppress anthracnose disease, structures of cucumber leaves infected with C. orbiculare were observed under a fluorescent microscope and a scanning electron microscope (SEM). Cucumber leaves pre-treated with bio-sulfur showed a low rate of appressorium formation whereas untreated ones showed abundant appressoria. Shrunk fungal hyphae were mostly observed on bio-sulfur-pretreated leaves by SEM. Similar results were observed on leaves pre-treated with a commercial fungicide Benomyl(R). These results suggest that inhibition of appressorium formation of C. orbiculare by bio-sulfur may contribute to its suppression of cucumber anthracnose.

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References

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