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Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur

  • Shin, Yong Ho (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Ko, Eun Ju (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Kim, Su Jeong (Postech Biotech Center, Pohang University of Science and Technology) ;
  • Hyun, He Nam (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University) ;
  • Jeun, Yong Chull (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
  • 투고 : 2019.04.17
  • 심사 : 2019.06.19
  • 발행 : 2019.10.01

초록

Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

키워드

참고문헌

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