Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of the Antioxidant Activities of Various Processed Fruits and Vegetables in APAP-induced Oxidative Stress in BALB/c Mice

  • Saba, Evelyn (Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Yuan Yee (Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Minki (Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Hyun-Kyoung (Department of Food Science and Engineering, Seowon University) ;
  • Rhee, Man Hee (Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2019.06.07
  • Accepted : 2019.07.04
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Research has established a strong connection between a diet rich in antioxidants and a decreased incidence of cardiovascular disease and cancer. These diets prominently feature fruits and vegetables containing high amounts of vitamins A, B, C and E, carotenoids, and minerals. Different processing conditions for these foods can alter their nutrient complement and potency. This study compared the antioxidant properties of a range of processed fruits and vegetables to see which yielded the highest level of antioxidant activity. We used an acetaminophen-induced oxidative stress mouse model to evaluate the antioxidant effects of extracts of processed apple, pear, carrot, cabbage, broccoli, and radish. Our results showed that the administration of these fruits decreased the expression of oxidative stress indicators such as ALT, AST, catalase, superoxide dismutase, GPx, and 8-OHdG. They also significantly protected mice livers from APAP-induced damage, as shown by histological evaluation. Our results have demonstrated the positive effects of processed fruits and vegetables in a mouse model of oxidative stress.

Keywords

References

  1. Alkadi H. A review on free radicals and antioxidants. Infect Disord Drug Targets. 2018. 10.2174/1871526518666180628124323:
  2. Blazka ME, Elwell MR, Holladay SD, Wilson RE, Luster MI. Histopathology of acetaminophen-induced liver changes: Role of interleukin $1{\alpha}$ and tumor necrosis factor $\alpha$. Toxicologic pathology. 1996. 24: 181-189. https://doi.org/10.1177/019262339602400206
  3. Boeing H, Bechthold A, Bub A, Ellinger S, Haller D, Kroke A, Leschik-Bonnet E, Muller MJ, Oberritter H, Schulze M, Stehle P, Watzl B. Critical review: Vegetables and fruit in the prevention of chronic diseases. Eur J Nutr. 2012. 51: 637-663. https://doi.org/10.1007/s00394-012-0380-y
  4. Casas R, Castro-Barquero S, Estruch R, Sacanella E. Nutrition and cardiovascular health. Int J Mol Sci. 2018. 19.
  5. Dauchet L, Amouyel P, Dallongeville J. Fruits, vegetables and coronary heart disease. Nat Rev Cardiol. 2009. 6: 599-608. https://doi.org/10.1038/nrcardio.2009.131
  6. Glorieux C, Calderon PB. Catalase, a remarkable enzyme: Targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem. 2017. 398: 1095-1108. https://doi.org/10.1515/hsz-2017-0131
  7. Huang X-J, Choi Y-K, Im H-S, Yarimaga O, Yoon E, Kim H-S. Aspartate aminotransferase (ast/got) and alanine aminotransferase (alt/gpt) detection techniques. Sensors. 2006. 6: 756-782. https://doi.org/10.3390/s6070756
  8. James LP, McCullough SS, Lamps LW, Hinson JA. Effect of nacetylcysteine on acetaminophen toxicity in mice: Relationship to reactive nitrogen and cytokine formation. Toxicol Sci. 2003. 75: 458-467. https://doi.org/10.1093/toxsci/kfg181
  9. Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci. 2015. 11: 982-991. https://doi.org/10.7150/ijbs.12096
  10. Kumar V, Khan AA, Tripathi A, Dixit PK, Bajaj U. Role of oxidative stress in various diseases: Relevance of dietary antioxidants. J Pharm Exp Ther. 2015. 4: 126-132.
  11. Lee YY, Saba E, Kim M, Rhee MH, Kim H-K. Antioxidant and anti-inflammatory properties of raw and processed fruits and vegetables. Biomed Sci Let. 2018. 24: 196-205. https://doi.org/10.15616/BSL.2018.24.3.196
  12. Leonard SS, Cutler D, Ding M, Vallyathan V, Castranova V, Shi X. Antioxidant properties of fruit and vegetable juices: More to the story than ascorbic acid. Annals of Clinical & Laboratory Science. 2002. 32: 193-200.
  13. Li S, Tan HY, Wang N, Cheung F, Hong M, Feng Y. The potential and action mechanism of polyphenols in the treatment of liver diseases. Oxid Med Cell Longev. 2018. 2018: 8394818.
  14. Liu Y, Fan C, Pu L, Wei C, Jin H, Teng Y, Zhao M, Yu AC, Jiang F, Shu J, Li F, Peng Q, Kong J, Pan B, Zheng L, Huang Y. Phloretin induces cell cycle arrest and apoptosis of human glioblastoma cells through the generation of reactive oxygen species. J Neurooncol. 2016. 128: 217-223. https://doi.org/10.1007/s11060-016-2107-z
  15. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010. 4: 118-126. https://doi.org/10.4103/0973-7847.70902
  16. Lubos E, Loscalzo J, Handy DE. Glutathione peroxidase-1 in health and disease: From molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal. 2011. 15: 1957-1997. https://doi.org/10.1089/ars.2010.3586
  17. Lutz M, Fuentes E, Avila F, Alarcon M, Palomo I. Roles of phenolic compounds in the reduction of risk factors of cardiovascular diseases. Molecules. 2019. 24.
  18. McGill MR. The past and present of serum aminotransferases and the future of liver injury biomarkers. EXCLI J. 2016. 15: 817-828.
  19. Miller HE, Rigelhof F, Marquart L, Prakash A, Kanter M. Antioxidant content of whole grain breakfast cereals, fruits and vegetables. Journal of the American College of Nutrition. 2000. 19: 312S-319S. https://doi.org/10.1080/07315724.2000.10718966
  20. Mossanen JC, Tacke F. Acetaminophen-induced acute liver injury in mice. Lab Anim. 2015. 49: 30-36. https://doi.org/10.1177/0023677215570992
  21. Northrop JH. The kinetics of the decomposition of peroxide by catalase. J Gen Physiol. 1925. 7: 373-387. https://doi.org/10.1085/jgp.7.3.373
  22. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009. 2: 270-278. https://doi.org/10.4161/oxim.2.5.9498
  23. Sharma S, Rana S, Patial V, Gupta M, Bhushan S, Padwad YS. Antioxidant and hepatoprotective effect of polyphenols from apple pomace extract via apoptosis inhibition and nrf2 activation in mice. Hum Exp Toxicol. 2016. 35: 1264-1275. https://doi.org/10.1177/0960327115627689
  24. Singh K, Singh N, Chandy A, Manigauha A. In vivo antioxidant and hepatoprotective activity of methanolic extracts of daucus carota seeds in experimental animals. Asian Pac J Trop Biomed. 2012. 2: 385-388. https://doi.org/10.1016/S2221-1691(12)60061-6
  25. Syed SN, Rizvi W, Kumar A, Khan AA, Moin S, Ahsan A. In vitro antioxidant and in vivo hepatoprotective activity of leave extract of raphanus sativus in rats using ccl4 model. Afr J Tradit Complement Altern Med. 2014. 11: 102-106. https://doi.org/10.4314/ajtcam.v11i3.15
  26. Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2'-deoxyguanosine (8-ohdg): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009. 27: 120-139. https://doi.org/10.1080/10590500902885684
  27. Younus H. Therapeutic potentials of superoxide dismutase. Int J Health Sci (Qassim). 2018. 12: 88-93.