Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

  • Park, Jun Seok (College of Veterinary Medicine, Chungnam National University) ;
  • Guevarra, Robin B. (Department of Animal Resources Science, Dankook University) ;
  • Kim, Bo-Ra (Department of Animal Resources Science, Dankook University) ;
  • Lee, Jun Hyung (Department of Animal Resources Science, Dankook University) ;
  • Lee, Sun Hee (Department of Animal Resources Science, Dankook University) ;
  • Cho, Jae Hyoung (Department of Animal Resources Science, Dankook University) ;
  • Kim, Hyeri (Department of Animal Resources Science, Dankook University) ;
  • Cho, Jin Ho (Division of Food and Animal Sciences, Chungbuk National University) ;
  • Song, Minho (Division of Animal and Dairy Science, Chungnam National University) ;
  • Lee, Ju-Hoon (Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Isaacson, Richard E. (Department of Veterinary and Biomedical Sciences, University of Minnesota) ;
  • Song, Kun Ho (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyeun Bum (Department of Animal Resources Science, Dankook University)
  • Received : 2019.01.21
  • Accepted : 2019.08.07
  • Published : 2019.09.28
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Abstract

Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

Keywords

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