International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Preparation and characterization of rutile phase TiO2 nanoparticles and their cytocompatibility with oral cancer cells

  • Vu, Phuong Dong (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University) ;
  • Nguyen, Thi Kieu Trang (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University) ;
  • Yoo, Hoon (Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University)
  • Received : 2019.08.16
  • Accepted : 2019.09.18
  • Published : 2019.09.30
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Abstract

In the present study, rutile phase titanium dioxide nanoparticles ($R-TiO_2$ NPs) were prepared by hydrolysis of titanium tetrachloride in an aqueous solution followed by calcination at $900^{\circ}C$. The composition of $R-TiO_2$ NPs was determined by the analysis of X-ray diffraction data, and the characteristic features of $R-TiO_2$ NPs such as the surface functional group, particle size, shape, surface topography, and morphological behavior were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy, transmission electron microscopy, dynamic light scattering, and zeta potential measurements. The average size of the prepared $R-TiO_2$ NPs was 76 nm, the surface area was $19m^2/g$, zeta potential was -20.8 mV, and average hydrodynamic diameter in dimethyl sulfoxide (DMSO)-$H_2O$ solution was 550 nm. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphological observations revealed that $R-TiO_2$ NPs were cytocompatible with oral cancer cells, with no inhibition of cell growth and proliferation. This suggests the efficacy of $R-TiO_2$ NPs for the aesthetic white pigmentation of teeth.

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References

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