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Development of suitable substrate of Sparassis latifolia for bottle cultivation

꽃송이버섯 병재배 적합 배지 개발

  • Gwon, Hee-Min (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Yun-Hae (Soil and Fertilizer Division, National Institute of Agricultural Sciences) ;
  • Choi, Jong-In (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Jeon, Dae-Hoon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Yong-seon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Young-Soon (Division of Environmental Agriculture Research, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services)
  • 권희민 (경기도농업기술원 버섯연구소) ;
  • 이윤혜 (국립농업과학원 토양비료과) ;
  • 최종인 (경기도농업기술원 버섯연구소) ;
  • 전대훈 (경기도농업기술원 버섯연구소) ;
  • 이용선 (경기도농업기술원 버섯연구소) ;
  • 이영순 (경기도농업기술원 환경농업과) ;
  • 김정한 (경기도농업기술원 버섯연구소)
  • Received : 2019.08.30
  • Accepted : 2019.09.23
  • Published : 2019.09.30

Abstract

This study sought to identify the optimum substrate composition for the stable bottle cultivation of Sparassis latifolia. The main substrate was fermented larch sawdust. Six nutrient sources were mixed at a maximum volume ratio of 20%. The fresh weight of fruit body was the highest at 128.5 g for GMSL69033 and 126.6 g for 'Neoul' in the treatments of beet pulp and corn flour in a volume ratio of 15:5. In addition, the total cultivation period was 94 days, which was shorter than that required for other treatments. The selected substrate characteristics were pH 4.7, C:N (carbon to nitrogen) ratio of 106:4, moisture content of 70%, and air filling porosity of 38%. We plan to develop new income items through research on mycelial incubation and fruit body growth conditions.

병재배에 적합한 꽃송이버섯 안정생산 재배기술 개발 연구를 위하여 낙엽송발효톱밥을 주배지로 하여 영양원으로 밀가루, 파옥쇄, 면실피, 비트펄프, 옥분, 옥피 6종을 혼합하여 시험을 수행하였다. 낙엽송발효톱밥+비트펄프+옥분(80:15:5, v/v) 처리에서 재배기간이 단축되고 수량이 높아 병재배에 적합한 배지로 선발하였다. 선발 배지의 균사배양 기간이 64일로 다른 처리에 비해 길었으나 균사배양과 원기형성이 동시에 진행됨에 따라 생육실로 입상하여 발생되는 기간이 단축됨으로써 생육 및 수확 기간이 단축되었다. 또한, 총 재배기간은 약 94일로 대조배지에 비해 약 17일정도 단축되었으며 첫 수확일부터 마지막 수확일까지 약 4일정도 소요됨으로써 수확기간이 짧아 재배사 운용효율을 높일 수 있을 것으로 생각된다. 수집균주인 GMSL69033 계통은 선발 배지에서 유의한 수준으로 수량이 높았다. 대조배지 104.3g에 반해 126.6g으로 약 18% 증가하였다. '너울' 품종에서 면실피와 옥분(15:5, v/v)처리와 선발한 배지에서 유의한 수준으로 수량이 높았으며 각각 121g, 128.5g이었다. 이 때 생물학적 효율은 면실피와 옥분(15:5, v/v) 배지에서 53.3%, 선발 배지에서 60.0%로 생물학적 효율 또한 높은 것을 확인할 수 있었다. 선발 배지에서 균주 간 차이 없이 수량이 우수한 것을 확인할 수 있었다. '너울' 품종에서는 선발 배지가 대조배지에 비해 수량이 약 21% 증가하였다. 이상의 결과 꽃송이 버섯 병재배 시 낙엽송발효톱밥+비트펄프+옥분(80:15:5, v/v)가 안정생산용 배지로 적합할 것으로 판단되며, 이때의 이화학성은 수분함량 70%, pH 4.7, C/N율 106.4, 공기충전공극률 38%이었다.

Keywords

References

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