융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

국화류 추출물 및 분획물의 생리활성 비교연구

Comparison of Biological Activities on Extracts and Fractions in Compositae Plants

  • 강정란 (우석대학교 제약공학과) ;
  • 유은미 (우석대학교 일반대학원 제약.화장품공학과) ;
  • 한갑훈 (우석대학교 제약공학과)
  • Kang, Jeong-Ran (Dept. of Pharmaceutical Engineering, Woosuk University) ;
  • Yu, Eun-Mi (Dept. of Pharmaceutical Cosmetics Engineering, Woosuk University) ;
  • Han, Kap-Hoon (Dept. of Pharmaceutical Engineering, Woosuk University)
  • 투고 : 2019.07.18
  • 심사 : 2019.09.20
  • 발행 : 2019.09.27
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 우리나라 전역에 자생하는 국화류3종 학슬, 희렴, 한련초를 대상으로 MeOH 80% 추출물 및 헥산, 클로로포름, 에틸아세테이트, 부탄올 및 물분획을 이용한 항균활성, 항산화 및 tyrosinase 저해활성효과에 대한 연구를 통해 기능성 화장품소재로서의 이용가능성을 확인하고자 하였다. 연구결과 항균활성의 경우 MeOH 80% 추출물에서는 한련초와 용매분획 중에서는 학슬의 헥산분획, 희렴의 클로로포름분획, 에틸아세테이트 분획, 한련초의 클로로포름분획을 중심으로 항균력을 나타내었다. 항산화 활성의 경우 3종 모두 용매분획 중 에틸아세테이트, 부탄올 분획에서 90%이상의 효과를 나타내었다. Tyrosinase 저해활성의 경우 희렴과 학슬의 에틸아세테이트 분획물에서 우수한 활성을 나타내었다. 이상의 국화류3종 MeOH 80%추출물 및 분획물에서 항산화 및 미백작용이 우수하므로 기능성화장품소재 개발 활용이 가능할 것으로 생각된다.

This study was conducted to investigate the anti-oxidative, anti-oxidative and tyrosinase inhibitory effects of MeOH 80% extract and hexane, chloroform, ethyl acetate, butanol and aqueous fraction on three kinds of compositae plants in Korea. In the antimicrobial effect, the extract and chloroform fraction of Eclipta prostrata and hexane fraction of Carpesium abrotanoides L. and chloroform fraction of Siegesbeckia glabrescens exhibited significant inhibition. The antioxidant activity of ethyl acetate and butanol fractions was more than 90% in all three plants. In case of tyrosinase activity, showed a potent inhibition ethlyacetate fraction of Siegesbeckia glabrescens and Carpesium abrotanoides L, which were higher than control group. In MeOH 80% extracts, there was not found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was ethylacetate fraction of Siegesbeckia glabrescens to show effectss commonly in the three assay system.

키워드

참고문헌

  1. H. W. Kang. (2012). Antioxidant and anti-inflammatory effect of extracts from Flammulina velutipes (Curtis) Singer. Journal of the Korean Society of Food Science and Nutrition, 41(8), 1072-1078. https://doi.org/10.3746/jkfn.2012.41.8.1072
  2. S. G. Lee, H. J. Jeong, B. J. Lee, J. B. Kim & S. W. Choi. (2011). Antioxidant and anti-inflammatory activities of ethanol extracts from medicinal herb mixture. Kor. J. Food Sci. Technol. 43, 200-205. https://doi.org/10.9721/KJFST.2011.43.2.200
  3. H. S. Kim, J. J. Ahn, T. H. Choi & T. Y. Hwang. (2014). Screening of DPPH radical scavenging and antimicrobial activity of extracts from local some native plants. Kor. J. Food Preserv. 21, 593-599. https://doi.org/10.11002/kjfp.2014.21.4.593
  4. T. J. Hemesath, E. R. Price, C. Takemoto, T. Badalian & D. E. Fisher. (1998). MAP kinase links the transcription factor Microphthalmia to c-kit signalling in melanocytes. Nature, 391, 298-301. https://doi.org/10.1038/34681
  5. E. S. Sohn, S. W. Kim, J. S. Kang & S. P. Lee. (2004). Technology trend and patent information analysis of cosmetic materials derived from natural products. Appl. Chem. 8, 466-469.
  6. E. C. Kim, S. Y. Ahn, E. S. Hong, G. H. Li, E. K. Kim & K. H. Row. (2005). Extraction of whitening agents from natural plants and whitening effect. Kor. J. Ind. Eng. Chem. 16, 348-353.
  7. A. Svobodova, J. Psotova & D. Walterova. (2003). Natural phenolics in the prevention of UV-induced skin damage a review. Biomed. Pap. 147, 137-145. https://doi.org/10.5507/bp.2003.019
  8. J. C. Fantone & P. A. Ward. (1982). Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am. J. Pathol. 107, 395-418.
  9. S. N. Park. (2003). Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes. Kor. J. Food. Sci. Technol. 35, 510-518.
  10. V. J. Hearing & M. Jimenez. (1987). Mammalian tyrosinase -the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19, 1141-1147. https://doi.org/10.1016/0020-711X(87)90095-4
  11. S. S. Choi. (2001). A study on the whitening substrate of natural products. Ph. D. Thesis, Chung-Ang University Seoul, korea.
  12. Y. C. Kim, D. J. Kim & C. M. Lee. (2004). Cosmetics Industry Dvelop Strategy. Korea Health Industry Development Institute. 18, 140-14.
  13. J. H. Woo, S. L. Shin & C. H. Lee. (2010). Antioxidant Effects of Ethanol Extracts from Flower Species of Compositae Plant. J. Korean Soc Food Sci Nutr 39(2), 159-164. https://doi.org/10.3746/jkfn.2010.39.2.159
  14. J. H, Woo & C. H. Lee. (2007). Antioxidative Effect of Extracts Obtained from Ten Compositae Species. The Korean Society of Plant Tissue Culture. 1, 345.
  15. T. Hatano, H. Kagawa & T. Okawa. (1988). Two new flavonoids and other constituents in licorice wet: their relative astringency and radical scavenging effects. Chem, Pharm, Bull., 36, 2090-2097. https://doi.org/10.1248/cpb.36.2090