Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Genomic characteristics of natural recombinant infectious bronchitis viruses isolated in Korea

  • Moon, Hyun-Woo (Laboratory of Veterinary Microbiology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Sung, Haan Woo (Laboratory of Veterinary Microbiology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kwon, Hyuk Moo (Laboratory of Veterinary Microbiology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • Received : 2019.04.19
  • Accepted : 2019.07.15
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Two infectious bronchitis virus (IBV) K046-12 and K047-12 strains were isolated and the nearly complete genomes of them were sequenced. Sequence comparisons showed that the K046-12 genome was most similar to Korean IBV strains, and the K047-12 genome was most similar to QX-like IBV strains. Phylogenetic analysis showed that nearly all K046-12 and most K046-12 genes were placed in the same cluster as Korean IBV isolates, but the S1 region was placed in the same cluster as Mass-type IBVs. For K047-12, nearly all K047-12 and most K047-12 genes were located in the same cluster as QX-like IBVs, but the M region was located in the same cluster as Korean IBV isolates with K047-12. Recombination analysis confirmed that K046-12 is a recombinant strain with the primary parental sequence derived from Korean IBVs and minor parental sequence derived from Mass-type IBV, and K047-12 is a recombinant strain with the major parental sequence derived from QX-IBV and minor parental sequence derived from Korean IBVs. This study showed that new IBV recombinants are constantly generated among various IBVs, including those used for vaccination. Therefore, genetic analysis of new virus isolates should be performed for effective infectious bronchitis control and appropriate vaccine development.

Keywords

References

  1. Jackwood MW, de Wit S. Infectious bronchitis. In: Diseases of Poultry. 13th ed. pp. 139-159, Iowa state University Press, Ames, 2013.
  2. Boursnell ME, Brown TD, Foulds IJ, Green PF, Tomley FM, Binns MM. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol 1987;68:57-77. https://doi.org/10.1099/0022-1317-68-1-57
  3. Siddell S, Wege H, Ter Meulen V. The biology of coronaviruses. J Gen Virol 1983;64:761-776. https://doi.org/10.1099/0022-1317-64-4-761
  4. Spaan W, Cavanagh D, Horzinek MC. Coronaviruses: structure and genome expression. J Gen Virol 1988;69:2939-2952. https://doi.org/10.1099/0022-1317-69-12-2939
  5. Brierley I, Boursnell ME, Binns MM, Bilimoria B, Blok VC, Brown TD, Inglis SC. An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO J 1987;6:3779-3785. https://doi.org/10.1002/j.1460-2075.1987.tb02713.x
  6. Ziebuhr J, Snijder EJ, Gorbalenya AE. Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 2000;81:853-879. https://doi.org/10.1099/0022-1317-81-4-853
  7. Armesto M, Cavanagh D, Britton P. The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity. PLoS One 2009;4:e7384. https://doi.org/10.1371/journal.pone.0007384
  8. Casais R, Dove B, Cavanagh D, Britton P. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J Virol 2003;77:9084-9089. https://doi.org/10.1128/JVI.77.16.9084-9089.2003
  9. Cavanagh D, Davis PJ, Cook JK, Li D, Kant A, Koch G. Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathol 1992;21:33-43. https://doi.org/10.1080/03079459208418816
  10. Koch G, Hartog L, Kant A, van Roozelaar DJ. Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. J Gen Virol 1990;71:1929-1935. https://doi.org/10.1099/0022-1317-71-9-1929
  11. Corse E, Machamer CE. The cytoplasmic tails of infectious bronchitis virus E and M proteins mediate their interaction. Virology 2003;312:25-34. https://doi.org/10.1016/S0042-6822(03)00175-2
  12. Casais R, Davies M, Cavanagh D, Britton P. Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication. J Virol 2005;79:8065-8078. https://doi.org/10.1128/JVI.79.13.8065-8078.2005
  13. Hodgson T, Britton P, Cavanagh D. Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication. J Virol 2006;80:296-305. https://doi.org/10.1128/JVI.80.1.296-305.2006
  14. Rhee YO, Kim JH, Kim JH, Mo IP, Youn HJ, Choi SH, Namgoong S. Outbreaks of infectious bronchitis in Korea. Korean J Vet Res 1986;26:277-282.
  15. Hong SM, Kwon HJ, Kim IH, Mo ML, Kim JH. Comparative genomics of Korean infectious bronchitis viruses (IBVs) and an animal model to evaluate pathogenicity of IBVs to the reproductive organs. Viruses 2012;4:2670-2683. https://doi.org/10.3390/v4112670
  16. Hong SM, Kwon HJ, Choi KS, Kim JH. Comparative genomics of QX-like infectious bronchitis viruses in Korea. Arch Virol 2017;162:1237-1250. https://doi.org/10.1007/s00705-016-3208-x
  17. Lim TH, Kim MS, Jang JH, Lee DH, Park JK, Youn HN, Lee JB, Park SY, Choi IS, Song CS. Live attenuated nephropathogenic infectious bronchitis virus vaccine provides broad cross protection against new variant strains. Poult Sci 2012;91:89-94. https://doi.org/10.3382/ps.2011-01739
  18. Mo ML, Hong SM, Kwon HJ, Kim IH, Song CS, Kim JH. Genetic diversity of spike, 3a, 3b and e genes of infectious bronchitis viruses and emergence of new recombinants in Korea. Viruses 2013;5:550-567. https://doi.org/10.3390/v5020550
  19. Song JE, Jeong WG, Sung HW, Kwon HM. Sequencing, phylogenetic analysis, and potential recombination events of infectious bronchitis viruses isolated in Korea. Virus Genes 2013;46:371-374. https://doi.org/10.1007/s11262-012-0856-0
  20. Jackwood MW, Boynton TO, Hilt DA, McKinley ET, Kissinger JC, Paterson AH, Robertson J, Lemke C, McCall AW, Williams SM, Jackwood JW, Byrd LA. Emergence of a group 3 coronavirus through recombination. Virology 2010;398:98-108. https://doi.org/10.1016/j.virol.2009.11.044
  21. Jang JH, Sung HW, Song CS, Kwon HM. Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea. J Vet Sci 2007;8:401-407. https://doi.org/10.4142/jvs.2007.8.4.401
  22. Lee EK, Jeon WJ, Lee YJ, Jeong OM, Choi JG, Kwon JH, Choi KS. Genetic diversity of avian infectious bronchitis virus isolates in Korea between 2003 and 2006. Avian Dis 2008;52:332-337. https://doi.org/10.1637/8117-092707-ResNote.1
  23. Lee SK, Sung HW, Kwon HM. S1 glycoprotein gene analysis of infectious bronchitis viruses isolated in Korea. Arch Virol 2004;149:481-494. https://doi.org/10.1007/s00705-003-0225-3
  24. Lim TH, Lee HJ, Lee DH, Lee YN, Park JK, Youn HN, Kim MS, Lee JB, Park SY, Choi IS, Song CS. An emerging recombinant cluster of nephropathogenic strains of avian infectious bronchitis virus in Korea. Infect Genet Evol 2011;11:678-685. https://doi.org/10.1016/j.meegid.2011.01.007
  25. Lee CW, Jackwood MW. Evidence of genetic diversity generated by recombination among avian coronavirus IBV. Arch Virol 2000;145:2135-2148. https://doi.org/10.1007/s007050070044
  26. Thor SW, Hilt DA, Kissinger JC, Paterson AH, Jackwood MW. Recombination in avian gamma-coronavirus infectious bronchitis virus. Viruses 2011;3:1777-1799. https://doi.org/10.3390/v3091777
  27. Zhang Y, Wang HN, Wang T, Fan WQ, Zhang AY, Wei K, Tian GB, Yang X. Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120. Virus Genes 2010;41:377-388. https://doi.org/10.1007/s11262-010-0517-0
  28. Zhao F, Zou N, Wang F, Guo M, Liu P, Wen X, Cao S, Huang Y. Analysis of a QX-like avian infectious bronchitis virus genome identified recombination in the region containing the ORF 5a, ORF 5b, and nucleocapsid protein gene sequences. Virus Genes 2013;46:454-464. https://doi.org/10.1007/s11262-013-0884-4
  29. Zhou S, Tang M, Jiang Y, Chen X, Shen X, Li J, Dai Y, Zou J. Complete genome sequence of a novel infectious bronchitis virus strain circulating in China with a distinct S gene. Virus Genes 2014;49:152-156. https://doi.org/10.1007/s11262-014-1063-y
  30. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC. Fulllength human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 1999;73:152-160. https://doi.org/10.1128/JVI.73.1.152-160.1999
  31. Denison MR, Graham RL, Donaldson EF, Eckerle LD, Baric RS. Coronaviruses: an RNA proofreading machine regulates replication fidelity and diversity. RNA Biol 2011;8:270-279. https://doi.org/10.4161/rna.8.2.15013
  32. Wang L, Junker D, Collisson EW. Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology 1993;192:710-716. https://doi.org/10.1006/viro.1993.1093
  33. Brooks JE, Rainer AC, Parr RL, Woolcock P, Hoerr F, Collisson EW. Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes. Virus Res 2004;100:191-198. https://doi.org/10.1016/j.virusres.2003.11.016
  34. Albanese GA, Lee DH, Cheng IN, Hilt DA, Jackwood MW, Jordan BJ. Biological and molecular characterization of ArkGA: a novel Arkansas serotype vaccine that is highly attenuated, efficacious, and protective against homologous challenge. Vaccine 2018;36:6077-6086. https://doi.org/10.1016/j.vaccine.2018.08.078