Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse

닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이

  • Cho, Jae-Keun (Metropolitan Health & Environmental Research Institute) ;
  • Son, Kyu-Hee (College of Veterinary Medicine, Kyoungpook National University) ;
  • Kim, Kyung-Hee (Metropolitan Health & Environmental Research Institute) ;
  • Kim, Jeong-Mi (Metropolitan Health & Environmental Research Institute) ;
  • Park, Dae-Hyun (Metropolitan Health & Environmental Research Institute) ;
  • Lee, Jung-Woo (Metropolitan Health & Environmental Research Institute)
  • 조재근 (대구광역시보건환경연구원) ;
  • 손규희 (경북대학교 수의과대학) ;
  • 김경희 (대구광역시보건환경연구원) ;
  • 김정미 (대구광역시보건환경연구원) ;
  • 박대현 (대구광역시보건환경연구원) ;
  • 이정우 (대구광역시보건환경연구원)
  • Received : 2019.09.09
  • Accepted : 2019.09.19
  • Published : 2019.09.30
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Abstract

The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

Keywords

References

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