한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제34권4호
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  • Pages.396-403
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  • 2019
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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연어(Oncorhynchus keta) 추출 anserine의 항산화 효과

Antioxidant Effect of Anserine Extracted from Salmon (Oncorhynchus keta)

  • 민혜옥 (식품의약품안전처 식품안전정책국 건강기능식품정책과) ;
  • 송호수 (영산대학교 서양조리학과)
  • Min, Hye-Ok (Ministry of Food and Drug Safety Food Safety Policy Bureau Health Functional Food Policy Division) ;
  • Song, Ho-Su (Department of Western Cuisine and Culinary Arts, Youngsan University)
  • 투고 : 2019.07.12
  • 심사 : 2019.07.17
  • 발행 : 2019.08.30
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초록

연어로부터 추출한 anserine의 항산화능 평가를 위해 1차 이온교환 후 한외여과 및 2차 이온교환 추출물인 IEC-B 실험구와 합성 anserine, ${\beta}-alanine$, 1-methylhistidine, taurine, ascorbic acid, BHT를 대조구로 DPPH 라디칼 소거능, 환원력, 금속 킬레이트능, SOD 유사활성 실험을 하였다. DPPH 라디칼 소거능 결과 대조구와 anserine 모두 농도 증가에 따라 라디칼 소거능이 증가하였으며 BHT가 가장 높은 소거능을 나타냈다. 합성 anserine은 농도 증가에 따라 $9.30{\pm}0.65{\sim}28.23{\pm}0.24%$를 나타냈으며 연어 추출 anserine인 IEC-B 추출물은 $7.30{\pm}1.13{\sim}31.05{\pm}0.17%$를 나타내 BHT, ascorbic acid에 비해 소거능이 낮으나 두 실험구와 비교했을 때 약 37%, 49% 정도의 소거능을 나타냈다. 환원력 또한 첨가농도에 따라 증가하였으며 BHT와 ascorbic acid가 높은 환원력을 나타냈고, 그 외 실험구 중에서는 합성 anserine과 연어 추출 anserine인 IEC-B 실험구가 높았으며 유의적으로 동일한 환원력을 나타냈다. 금속 킬레이트능은 BHT, ascorbic acid 순으로 높았으며 합성 anserine과 연어 추출 anserine의 경우 ascorbic acid와 유사한 금속 킬레이트능을 나타냈다. 또한 SOD 유사활성에서는 연어 추출 anserine이 BHT와 ascorbic acid에 비해 낮았으나 농도 증가에 따라 증가하는 경향을 나타내었으며, 합성 anserine, 연어 추출 anserine, ascorbic acid, BHT를 이용하여 과산화물가와 TBARS를 실험하였다. 저장기간 중 linoleic acid의 과산화물가는 큰 폭으로 증가한 반면 연어 추출 anserine을 첨가한 경우 과산화물가의 증가가 억제되는 경향이 나타났으며, 이는 TBARS 실험 결과와 유사한 경향을 나타냈다.

Ion-exchange chromatography and ultrafiltration were used to extract anserine from salmon (Oncorhynchus keta). The salmon anserine showed DPPH radical scavenging activity in the range of 7.30% to 31.05% in a dose-dependent manner. This reducing power of salmon anserine also increased as the concentration increased. Metal chelate activity, superoxide dismutase - like activity, and thiobarbituric acid reactive substances assay showed similar results. The anserine also suppressed the increment of the peroxide value and linoleic acid during storage periods. These results suggest that salmon anserine might be useful as a natural antioxidant in various foodstuffs.

키워드

참고문헌

  1. Auh, J.H, Namgung, N, Shin, K.S., Park, S.W., Paik, I.K.: Effects of supplementary blood meal on the content of carnosine and anserine in broiler meat. J. Poult Sci. 47, 302-309 (2010). https://doi.org/10.2141/jpsa.010024
  2. Marit, A., Lief, J, Hans, G.: Quantitative high-resolution 13C nuclear magnetic resonance of anserine and lactate in white muscle of Atlantic salmon(Salmo salar). Comp Biochem Physiol. 112B, 315-321 (1995).
  3. Suyama, M., Suzuki, T., Nonaka, J.: Chromatographic determination of imidazole compounds in the whale meat. Bulletin of the Japanese Society of Scientific Fisheries. 33, 141-146 (1967). https://doi.org/10.2331/suisan.33.141
  4. Abe, H., Okuma, E.: Effect of temperature on the buffering capacities of histidine-related compounds and fish skeletal muscle. J. Jap. Fish Soc. 57, 2101-2107 (1991).
  5. Huang, S.C., Kuo, J.C.C.: Concentration and antioxidative activity of anserine and carnosine in poultry meat extracts treated with demineralization and papain. Proc. Natl. Sci. Counc. Repub China part B Life Sci. 24, 193- 201 (2000).
  6. Wu, H.C., Shiau, C.Y., Chen, H.M., Chiou, T.K.: Antioxidant activities of carnosine, anserine, some free amino acids and their combination. J. Food Drug Anal. 11, 148-153 (2003).
  7. Lee, K.T., Song, H.S., Park, S.M.,: Antioxidant activities of carnosine, extracted from eel (Anguilla japonica) J. Korean Fish Soc. 40, 193-200 (2007).
  8. McCord, J.M., Fridovich, I.: Superoxide dismutase. An enzymic function for erythrocuprein(hemocuprein). J. Biol. Chem. 244, 6049-6055 (1969). https://doi.org/10.1016/S0021-9258(18)63504-5
  9. Frankel, E.N.: Antioxidants in lipid foods and their on food quality . Food Chem., 57, 51-55 (1996). https://doi.org/10.1016/0308-8146(96)00067-2
  10. Giese, J.: Antioxidants : Tool for preventing lipid oxidation. Food Technol., 50, 73-81 (1996).
  11. Ito, N., Fukushima, S., Hasegawa,A., ShibataM., Ogiso. T.,: Carcinogenicity of bytylated hydroxy antisole in 344 rats. J. Natl. Cancel Inst., 70, 343-352 (1983).
  12. Branen, A.L.: Toxicological and anisole and butylated hydroxy antisole and butylated hy-droxytoluene. JAOCS, 52, 59 (1975). https://doi.org/10.1007/BF02901825
  13. Larson, R.A.: The antioxidants of higher plants. phycochemistry, 27, 969-978 (1988). https://doi.org/10.1016/0031-9422(88)80254-1
  14. Partt, D.E., Hudson, B.J.F.: Natural antioxidant not exploited commercially. In: Food Antioxidants. Elsevier, 168-171 (1990).
  15. Burton, G.W.: Antioxidant action of cartenoids. J. Nutri., 119, 110-116 (1989).
  16. Block, G. Langseth, L.: Antioxidant vitamins and disease prevention. Food Technol., 48, 80-85 (1994).
  17. Decker, E.A., Crum, A.D.: Antioxidant activity of carnosine in cooked ground pork. Meat sci., 34, 245-253 (1993). https://doi.org/10.1016/0309-1740(93)90031-C
  18. Casteels, P., Ampe, C., Tempst, P.: Antibacterial peptides from honeybees. EMBOJ, 9, 2397-2391 (1989).
  19. Nakazato, M., Asai, J. Mitazato, M.,Matsuo, H.: Isolation and identification of isletamyloid polypeptide in normal human pancrease. Regul. peptides, 31, 179-186 (1990). https://doi.org/10.1016/0167-0115(90)90004-G
  20. Seki, E.K. Osajima, T, Matsui, Osajima, Y.: Separation and purification of angiotensin I converting enzyme inhibitory peptides from heated sardine meat by treatment with alkaline protease. Nippon Shokuhin Hogyo Gakkashi, 40, 783-791 (1993). https://doi.org/10.3136/nskkk1962.40.783
  21. Kohama, Y.: Isolation of angiotensin converting enzyme inhibitor from tuna muscle. Biochem. Biophy. Res. Commun. 155, 332-336 (1988). https://doi.org/10.1016/S0006-291X(88)81089-1
  22. Kim, S.K., Lee, H.C., Jeon, Y.J.: Isolation and characterization of antioxidative peptides from enzymatic hydrolysates of yellowfin sole skin gelatin. J. Kor. Fish. Soc., 29, 246-255 (1996).
  23. Kim, S.K., Choi,Y.I., Choi., J.H.: Screening of biofuntional pepteides from cod processing wastes. J. Kor. Soc. Agric. Chem. Biotechnol., 43, 225-227 (2000).
  24. Nam, H.S.: Development of bioactive peptides and its market trend. Food Ind. Nutri., 4, 17-19 (1999).
  25. Suyama, M., Hinaro, T., Suzuki, T.: Buffering capacity of free histidine and its related dipeptides in white and dark muscle of yellowfin tuna. Bull. Jpn. Soc. Sci. Fish., 52, 2171-2175 (1986). https://doi.org/10.2331/suisan.52.2171
  26. Chan, K.M., Decker, E., Means, W.J.: Extraction and activity of carnosine a natually occuring antioxidant in beef muscle. J. Food Sci. 58, 1-7 (1993). https://doi.org/10.1111/j.1365-2621.1993.tb03199.x
  27. Wu, H.C., Shiau, C.Y., Chen, H.M., Chiou, T.K.: Antioxidant activities of carnosine, anserine, some free amino acids and their combination. J. Food Drug Anal 11, 148-153 (2003).
  28. Oyaizu, M.: Antioxidative activities of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. J. Jap. Fish. Soc., 35, 771-775 (1988).
  29. Decker, E.A. Welch, B.: Role of ferritin as a lipid oxidation catalyst in muscle food. J. Agric. Food Chem., 38, 674-677 (1990). https://doi.org/10.1021/jf00093a019
  30. Marklund, S. Marklund, G.: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 469-474 (1974). https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  31. A.O.A.C.: Official methods of analysis. 15th ed., Association of Official Analytical Chemists, Washington D. C. USA, 777-788.(1995).
  32. Mitsuda, H., Yasumoto, K. Iwami, K.: Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo To Shlkuryo., 19, 210-217 (1966). https://doi.org/10.4327/jsnfs1949.19.210
  33. Sidwell, C.G., Salwin,H., Benca, M., Mitchell, J.H., Jr.: The use of thiobarbituric acid as a measure of fat oxidation. J. Am. Oil. Chem. Soc., 31, 603-609 (1954). https://doi.org/10.1007/BF02545589
  34. Son, J.Y., Rhim, J.H., Son, H.S.: Effect of some synthetic and natural antioxidants on the oxidative stability of skip jack oil. Kor. J. Food Nutr., 8, 88-92 (1995).
  35. Kazuaki, K., Yoshiharu, M., Kazuo, S.: Sepatation and physiological functions of anserine from fish extract. Develop. in Food Sci., 42, 97-105 (2004). https://doi.org/10.1016/S0167-4501(04)80012-8
  36. Qi, H., Zhang, Q., Zhao, T., Hu, R., Zhang, K., Li, Z.: Antioxidant activity of acetylated and benzoylated derivatives of polysacccharide extrated from Ulva pertusa (Chlorophyta). Bioorgan. and Medic. Chem. Letters, 16, 2441-2445 (2006). https://doi.org/10.1016/j.bmcl.2006.01.076
  37. Gordon, M.H.: The machanism of antioxidant action in vitro. In food antioxidants. Hudson BJF, ed. Elsevier Applied Sci., London/New York. 1-18 (1990).
  38. Decker, E.A., Hultin, H.O.: Lipid oxidation in muscle foods via redox iron. In A. J. St. Angelo (Ed.), Lipid oxidation in food (Chapter 3); ACS Symposium sereis 500, Washington, DC. American Chemical Society (1992).
  39. Stoch, S.J. Bagchi, D.: Oxidative mechanisms in the toxicity of metal ions. Free Radical Biol. Medic., 18, 321-336 (1995). https://doi.org/10.1016/0891-5849(94)00159-H
  40. Pokorny, J., Yanishlieva, N., Gordon, M.: Antioxidants in food. CRC., 71-83. (2001).
  41. Kim, W.S.: Antioxidant activity and safety evaluation of carnosine, anserine and homocarnosine isolated from Spent hen. Ph.D. Thesis, Duksung Women's University, Seoul, Korea, 1-94 (2003).
  42. Wu, H.C., Shiau, C.Y., Chen, H.M., Chiou, T.K.: Antioxidant activities of carnosine, anserine, some free amino acids and their combination. J. of Food and Drug Anal., 11, 148-153 (2003).
  43. Boldyrev, A.H., Ave, S., Stvolinsky, Tyulina, O.: Effects of carnosine and related compounds on generation of free oxygen species : A comparatibe study. Comp. Biochem. Physiol., 112, 481-485 (1995). https://doi.org/10.1016/0305-0491(95)00084-4
  44. Pokorny, J., Yanishlieva, N., Gordon, M.: Antioxidants in food. CRC., 71-83 (2001)
  45. Laleye, L.C., Lee, B.H., Simard, R.E., Camichael, L., Holly, R.A.: Shelf life of vacuum-or nitrogen packed pastrami: effects of packaging atomspheres, temperature and duration of storage on microflora changes. J. Food. Sci., 49, 827-831 (1984). https://doi.org/10.1111/j.1365-2621.1984.tb13222.x