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Heat-Killed Lactobacillus plantarum KCTC 13314BP Enhances Phagocytic Activity and Immunomodulatory Effects via Activation of MAPK and STAT3 Pathways

  • Jeong, Minju (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jae Hwan (Department of Agricultural Biotechnology, Seoul National University) ;
  • Yang, Hee (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kang, Shin Dal (Research Institute of Food and Biotechnology, SPC Group) ;
  • Song, Seongbong (Research Institute of Food and Biotechnology, SPC Group) ;
  • Lee, Deukbuhm (Research Institute of Food and Biotechnology, SPC Group) ;
  • Lee, Ji Su (Division of Bioengineering, Incheon National University) ;
  • Park, Jung Han Yoon (Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Byun, Sanguine (Division of Bioengineering, Incheon National University) ;
  • Lee, Ki Won (Department of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2019.05.30
  • 심사 : 2019.06.13
  • 발행 : 2019.08.28

초록

Identification of novel probiotic strains is of great interest in the field of functional foods. Specific strains of heat-killed bacteria have been reported to exert immunomodulatory effects. Herein, we investigated the immune-stimulatory function of heat-killed Lactobacillus plantarum KCTC 13314BP (LBP). Treatment with LBP significantly increased the production of $TNF-{\alpha}$ and IL-6 by macrophages. More importantly, LBP was able to enhance the phagocytic activity of macrophages against bacterial particles. Activation of p38, JNK, ERK, $NF-{\kappa}B$, and STAT3 was involved in the immunomodulatory function of LBP. LBP treatment significantly increased production of $TNF-{\alpha}$ by bone marrow-derived macrophages and splenocytes, further confirming the immunostimulatory effect of LBP in primary immune cells. Interestingly, the immunomodulatory effects of LBP were much stronger than those of Lactobacillus rhamnosus GG, a well-known probiotic strain. These results indicate that LBP can be a promising immune-enhancing functional food agent.

키워드

참고문헌

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