Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Protective Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against Hydrogen Peroxide-Induced Oxidative Stress In Vitro and In Vivo

  • Cho, Su-Hyeon (Chuncheon Center, Korea Basic Science Institute (KBSI)) ;
  • Heo, Soo-Jin (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science and Technology) ;
  • Yang, Hye-Won (Department of Marine Life Science, Jeju National University) ;
  • Ko, Eun-Yi (Bio Research Center) ;
  • Jung, Myeong Seon (Chuncheon Center, Korea Basic Science Institute (KBSI)) ;
  • Cha, Seon-Heui (Department of Marine Biomedical Science, Hanseo University) ;
  • Ahn, Ginnae (Department of Marine Bio-food Science, College of Fisheries and Ocean Sciences, Chonnam National University) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University) ;
  • Kim, Kil-Nam (Chuncheon Center, Korea Basic Science Institute (KBSI))
  • Received : 2019.04.29
  • Accepted : 2019.07.02
  • Published : 2019.08.28
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Abstract

We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide ($H_2O_2$)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited $H_2O_2$-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and $NF-{\kappa}B$ was assessed by western blot assay. BDB significantly suppressed the cleavage of caspase-9 and PARP and reduced Bax levels in $H_2O_2$-induced Vero cells. Besides, BDB suppressed the phosphorylation of $NF-{\kappa}$B and the translocation of p65 in $H_2O_2$-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell death, and lipid peroxidation in an $H_2O_2$-stimulated zebrafish embryo model. Taken together, these results indicated that ROS generation and cell death were significantly inhibited by BDB in zebrafish embryos, thereby proving that BDB exerts excellent antioxidant activity in vitro and in vivo.

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