Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Production and development of porcine tetraploid parthenogenetic embryos

  • Lin, Tao (Division of Animal & Dairy Science, Chungnam National University) ;
  • Lee, Jae Eun (Division of Animal & Dairy Science, Chungnam National University) ;
  • Shin, Hyeon Yeong (Division of Animal & Dairy Science, Chungnam National University) ;
  • Lee, Joo Bin (Division of Animal & Dairy Science, Chungnam National University) ;
  • Kim, So Yeon (Division of Animal & Dairy Science, Chungnam National University) ;
  • Jin, Dong Il (Division of Animal & Dairy Science, Chungnam National University)
  • Received : 2019.06.26
  • Accepted : 2019.07.02
  • Published : 2019.07.31
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Abstract

The aim of this study was to produce porcine tetraploid (4N) parthenogenetic embryos using various methods and evaluate their developmental potential. In method 1 (M1), porcine 4N parthenogenetic embryos were obtained by inhibiting extrusion of both first (PB1) and second (PB2) polar bodies; in methods 2 (M2) and 3 (M3), 4N parthenogenetic embryos were obtained by electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 or PB1 extrusion, respectively. We found no differences in the rates of cleavage or blastocyst formation or the proportion of 4N embryos among M1, M2, and M3 groups. The different methods also did not influence apoptosis rates (number of TUNEL-positive cells/number of total cells) or expression levels of apoptosis-related BAX and BCL2L1 genes. However, total cell and EdU (5-ethynyl-2'-deoxyuridine)-positive cell numbers in 4N parthenogenetic blastocysts derived from M1 were higher (p < 0.05) than those for M2 and M3 groups. Our results suggest that, although porcine 4N parthenogenetic embryos could be produced by a variety of methods, inhibition of PB1 and PB2 extrusion (M1) is superior to electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 (M2) or PB1 (M3) extrusion.

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References

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