Mycobiology

  • 제47권2호
  • /
  • Pages.256-260
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  • 2019
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  • 1229-8093(pISSN)
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  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Neuraminidase Inhibitors from the Fruiting Body of Glaziella splendens

  • Kim, Ji-Yul (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Woo, E-Eum (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Ha, Lee Su (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Ki, Dae-Won (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University)
  • 투고 : 2019.03.06
  • 심사 : 2019.05.05
  • 발행 : 2019.06.01
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초록

Neuraminidase (NA) cleaves the glycosidic bond linkages of sialic acids to release the mature virions from infected cells and has been an attractive therapeutic target for anti-influenza agents. In our ongoing investigation of NA inhibitors in mushroom extracts, we found that the extract the fruiting body of Glaziella splendens potently inhibited neuraminidase. The fruiting bodies of G. splendens were extracted and partitioned successively with hexane, ethyl acetate, and butanol. The ethyl acetate soluble-layer was subjected to silica gel and Sephadex LH-20 column chromatographies, and MPLC to obtain five compounds (1-5). Their structures were determined by spectroscopic methods. NA inhibitory activity of these compounds was evaluated using NAs from recombinant rvH1N1, H3N2, and H5N1 influenza A viruses. One compound (1) was elucidated as a new azaphilone derivative, and four compounds (2-5) were identified as entonaemin A, comazaphilone D, rubiginosin A, and entonaemin B, respectively. Compounds 3 and 4 showed considerable inhibitory activity against three types of neuraminidases with the $IC_{50}$ values of 30.9, 41.8, and $35.7{\mu}M$ for 3 and 46.5, 50.4, and $29.9{\mu}M$ for 4, respectively. This study reveals that the fruiting bodies of G. splendens possess azaphilone derivatives with the NA inhibitory activity. This is the first report on the isolation of neuraminidase inhibitors from the fruiting bodies of G. splendens.

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참고문헌

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피인용 문헌

  1. Recent advances in the chemistry and biology of azaphilones vol.10, pp.17, 2019, https://doi.org/10.1039/d0ra00894j
  2. Prospects for the Development of Anti-Influenza Drugs Based on Medicinal Mushrooms (Review) vol.56, pp.5, 2019, https://doi.org/10.1134/s0003683820050142
  3. Recent Findings in Azaphilone Pigments vol.7, pp.7, 2021, https://doi.org/10.3390/jof7070541