한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제23권4호
  • /
  • Pages.385-390
  • /
  • 2019
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Generation of mmp15b Zebrafish Mutant to Investigate Liver Diseases

  • Kim, Oc-Hee (Dept. of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • An, Hye Suck (Dept. of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Choi, Tae-Young (Dept. of Genetic Resources Research, National Marine Biodiversity Institute of Korea)
  • 투고 : 2019.10.18
  • 심사 : 2019.11.09
  • 발행 : 2019.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Upon gene inactivation in animal models, the zebrafish (Danio rerio) has become a useful model organism for many reasons, including the fact that it is amenable to various forms of genetic manipulation. Genome editing is a type of genetic engineering in which DNA is inserted, deleted, modified, or replaced in the genome of a living organism. Mainly, CRISPR (clustered regularly interspaced short palindromic repeats) Cas9 (CRISPR-associated protein 9) is a technology that enables geneticists to edit parts of the genome. In this study, we utilized this technology to generate an mmp15b mutant by using zebrafish as an animal model. MMP15 is the membrane-type MMP (MT-MMP) which is a recently identified matrix metalloproteinase (MMP) capable of degrading all kinds of extracellular matrix proteins as well as numerous bioactive molecules. Although the newly-established mmp15b zebrafish mutant didn't exhibit morphological phenotypes in the developing embryos, it might be further utilized to understand the role of MMP15 in liver-related diseases, such as liver fibrosis, and associated pathogeneses in humans.

키워드

참고문헌

  1. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG, Tan W, Penheiter SG, Ma AC, Leung AYH, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC (2012) In vivo genome editing using a high-efficiency TALEN system. Nature 491:114- 118. https://doi.org/10.1038/nature11537
  2. Choi TY, Khaliq M, Tsurusaki S, Ninov N, Stainier DYR, Tanaka M, Shin D (2017) Bone morphogenetic protein signaling governs biliary-driven liver regeneration in zebrafish through tbx2b and id2a. Hepatology 66:1616- 1630. https://doi.org/10.1002/hep.29309
  3. Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK (2014) Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol 32:279-284. https://doi.org/10.1038/nbt.2808
  4. Giannandrea M, Parks WC (2014) Diverse functions of matrix metalloproteinases during fibrosis. Dis Model Mech 7:193-203. https://doi.org/10.1242/dmm.012062
  5. Hsu PD, Lander ES, Zhang, F (2014) Development and applications of CRISPR-Cas9 for genome engineering. Cell 157:1262-1278. https://doi.org/10.1016/j.cell.2014.05.010
  6. Irion U, Krauss J, Nusslein-Volhard C (2014) Precise and efficient genome editing in zebrafish using the CRISPR/ Cas9 system. Development 141:4827-4830. https://doi.org/10.1242/dev.115584
  7. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816-821. https://doi.org/10.1126/science.1225829
  8. Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK (2016) High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature 529:490-495. https://doi.org/10.1038/nature16526
  9. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature 523:481-485. https://doi.org/10.1038/nature14592
  10. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM (2013) RNA-guided human genome engineering via Cas9. Science 339:823-826. https://doi.org/10.1126/science.1232033
  11. Ra HJ, Parks WC (2007) Control of matrix metalloproteinase catalytic activity. Matrix Biology 26:587-596. https://doi.org/10.1016/j.matbio.2007.07.001
  12. Varshney GK, Sood R, Burgess SM (2015) Understanding and editing the zebrafish genome. Adv Genet 92:1-52. https://doi.org/10.1016/bs.adgen.2015.09.002
  13. Westerfield M (2000) The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio). University of Oregon Press, Eugene.
  14. Yoshifum I (2015) Membrane-type matrix metalloproteinases: Their functions and regulations. Matrix Biol 44-46: 207-223. https://doi.org/10.1016/j.matbio.2015.03.004