International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions

  • Lim, Jung Jin (Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Kim, Hyung Joon (Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Rhie, Byung-ho (Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Lee, Man Ryul (Soonchunhyang Institute of Medi-bioscience, Soonchunhyang University) ;
  • Choi, Myeong Jun (1st Research Center, Axceso Biopharma Co., Ltd.) ;
  • Hong, Seok-Ho (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Kim, Kye-Seong (Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University)
  • Received : 2019.07.19
  • Accepted : 2019.09.16
  • Published : 2019.11.30
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Abstract

Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have established a variety of culture systems in a defined combination, xeno-free matrix and medium that supports the growth and differentiation of hPSCs. Here we described detailed hPSCs culture methods under feeder-free and chemically defined conditions using vitronetin and TeSR-E8 medium including supplement bioactive lysophospholipid for promoting hPSCs proliferation and maintaining stemness.

Keywords

Acknowledgement

This research was supported by a grant from the Korea Centers for Disease Control and Prevention (2017ER610300) and the Bio and Medical Technology Development Program (2017M3A9B3061830) of the National Research Foundation (NRF). We are also grateful to Axceso Biopharma Co., Ltd. for providing cP1P and for excellent technical assistance.

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