Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Carpinus turczaninowii extract modulates arterial inflammatory response: a potential therapeutic use for atherosclerosis

  • Son, Youn Kyoung (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Yoon, So Ra (Department of Food Science and Nutrition, Dong-A University) ;
  • Bang, Woo Young (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Bae, Chang-Hwan (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Yeo, Joo-Hong (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Yeo, Rimkyo (Department of Food Science and Nutrition, Dong-A University) ;
  • An, Juhyun (Department of Food Science and Nutrition, Dong-A University) ;
  • Song, Juhyun (Departments of Anatomy, Chonnam National University Medical School) ;
  • Kim, Oh Yoen (Department of Food Science and Nutrition, Dong-A University)
  • Received : 2018.12.16
  • Accepted : 2019.05.14
  • Published : 2019.08.01
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Abstract

BACKGOURND/OBJECTIVES: Vascular inflammation is an important feature in the atherosclerotic process. Recent studies report that leaves and branches of Carpinus turczaninowii (C. turczaninowii) have antioxidant capacity and exert anti-inflammatory effects. However, no study has reported the regulatory effect of C. turczaninowii extract on the arterial inflammatory response. This study therefore investigated modulation of the arterial inflammatory response after exposure to C. turczaninowii extract, using human aortic vascular smooth muscle cells (HAoSMCs). MATERIALS/METHODS: Scavenging activity of free radicals, total phenolic content (TPC), cell viability, mRNA expressions, and secreted levels of cytokines were measured in LPS-stimulated (10 ng/mL) HAoSMCs treated with the C. turczaninowii extract. RESULTS: C. turczaninowii extract contains high amounts of TPC ($225.6{\pm}21.0mg$ of gallic acid equivalents/g of the extract), as well as exerts time-and dose-dependent increases in strongly scavenged free radicals (average $14.8{\pm}1.97{\mu}g/mL$ $IC_{50}$ at 40 min). Cell viabilities after exposure to the extracts (1 and $10{\mu}g/mL$) were similar to the viability of non-treated cells. Cytokine mRNA expressions were significantly suppressed by the extracts (1 and $10{\mu}g/mL$) at 6 hours (h) after exposure. Interleukin-6 secretion was dose-dependently suppressed 2 h after incubation with the extract, at $1-10{\mu}g/mL$ in non-stimulated cells, and at 5 and $10{\mu}g/mL$ in LPS-stimulated cells. Similar patterns were also observed at 24 h after incubation with the extract (at $1-10{\mu}g/mL$ in non-stimulated cells, and at $10{\mu}g/mL$ in the LPS-stimulated cells). Soluble intracellular vascular adhesion molecules (sICAM-1) secreted from non-stimulated cells and LPS-stimulated cells were similarly suppressed in a dose-dependent manner after 24 h exposure to the extracts, but not after 2 h. In addition, sICAM-1 concentration after 24 h treatment was positively related to IL-6 levels after 2 h and 24 h exposure (r = 0.418, P = 0.003, and r = 0.524, P < 0.001, respectively). CONCLUSIONS: This study demonstrates that C. turczaninowii modulates the arterial inflammatory response, and indicates the potential to be applied as a therapeutic use for atherosclerosis.

Keywords

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