Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro mass propagation and acclimatization of Haworthia truncata

하월시아 옥선(Haworthia truncata)의 기내 대량 증식 및 순화 조건 구명

  • Kim, Youn Hee (Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Lee, Gee Young (Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Kim, Hye Hyeong (Horticultural Research Division) ;
  • Lee, Jae Hong (Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Jung, Jae Hong (Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Lee, Sang Deok (Cactus and Succulents Research Institute, Gyeonggi-do Agricultural Research and Extension Services)
  • 김윤희 (경기도농업기술원 선인장다육식물연구소) ;
  • 이지영 (경기도농업기술원 선인장다육식물연구소) ;
  • 김혜형 (경기도농업기술원 원예연구과) ;
  • 이재홍 (경기도농업기술원 선인장다육식물연구소) ;
  • 정재홍 (경기도농업기술원 선인장다육식물연구소) ;
  • 이상덕 (경기도농업기술원 선인장다육식물연구소)
  • Received : 2019.04.13
  • Accepted : 2019.05.17
  • Published : 2019.06.30
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Abstract

The purpose of this study was to investigate suitable parts for callus induction and optimal concentrations of growth regulators, contained in the medium affecting shoot and rooting for in vitro mass production of Haworthia truncata. Leaves and flower bud showed 100% callus formation rate at NAA $1{\sim}2mgL^{-1}$ treatment, and NAA $1mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment. The flower stalk showed 75% callus formation rate, at NAA $2mgL^{-1}$ + TDZ $2mgL^{-1}$ treatment in H. truncata. While the rate of callus formation was high in leaves and flower bud, leaves were the most efficient in obtaining most culture parts. Shoot induction rate from callus was highest, at NAA $0.1mgL^{-1}$ treatment in H. truncata. Additionally, the number of shoots formation was 66.3 shoots high, in NAA $1mgL^{-1}$ + BA $0.1mgL^{-1}$ treatment in H. truncata. In the case of acclimatization of regenerated plant, growth characteristics did not show significant difference (95%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate, considering plant height and appearance preference of H. truncata. It was established that optimization of culture condition, was responsible for mass propagation in vitro cultures of H. truncata.

하월시아 옥선(Haworthia truncata)의 기내 대량생산을 위하여 캘러스 유도에 적합한 부위와 기내 재분화 조건을 확립하고, 형성된 식물체의 기외 순화조건을 구명하고자 본 연구를 수행하였다. 캘러스를 유도에 적합한 식물 부위를 구명하기 위해 잎, 화뢰, 화경을 이용하여 NAA와 TDZ의 농도를 달리하여 MS배지에 배양한 결과, 잎은 NAA $1{\sim}2mgL^{-1}$ 단용 배지, 화뢰 NAA $1mgL^{-1}$ + TDZ $2mgL^{-1}$혼용처리에서 100% 캘러스 형성율을 보였으며, 화경 NAA $2mgL^{-1}$ + TDZ $2mgL^{-1}$혼용처리에서 75% 캘러스 형성율을 나타냈으며 이 중 가장 많은 배양 절편체 확보가 가능한 잎이 옥선(H. truncata)의 대량 증식에 가장 효율적일 것으로 판단되었다. Shoot 유도 배지로는 NAA $0.1mgL^{-1}$의 단용 처리구에서 다른 처리에 비해서 shoot가 24.2개로 shoot 발생이 가장 많았으며, shoot 증식(multiple shoot)은 NAA $1mgL^{-1}$ + BA $0.1mgL^{-1}$ 혼용 처리구에서 multiple shoot가 66.3개로 가장 많이 형성되었다. 재분화 식물체의 기외 순화조건은 용토에 따라 생육에 큰 차이를 보이지 않았으나 식물체의 고유 엽색 유지, 엽장, 생존율을 고려하여 차광막 95%에서 순화 처리하는 것이 적합할 것으로 판단되었다. 이러한 결과는 최적의 기내 배양 조건 및 재분화 식물체 순화 조건 구명을 통해 번식이 어려운 옥선(H. truncata)의 균일하고 안정적인 대량생산이 가능할 것으로 기대된다.

Keywords

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