Journal of Plant Biotechnology

  • 제46권2호
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  • Pages.88-96
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  • 2019
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Physiological and molecular analysis of OsTPS30 by gamma irradiation

  • Kim, Se Won (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)) ;
  • Jung, In Jung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)) ;
  • Choi, Hong-Il (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI))
  • 투고 : 2019.02.01
  • 심사 : 2019.03.29
  • 발행 : 2019.06.30
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초록

Terpenes constitute a large class of secondary metabolites in plants. The Oryza sativa terpene synthase is a vital gene in plant defense response. In this study, the molecular and physiological functions of Oryza sativa terpene synthase 30 (OsTPS30, LOC_Os08g07080) were investigated after exposure of the seeds and plants to gamma-rays. The OsTPS30 expression was slightly induced at 200 Gray (Gy), but was significantly induced at 400 Gy. The total terpenoid was synthesized more in OsTPS30-overexpressing (OX-OsTPS30) Arabidopsisthaliana plants than in wild-type (WT) plants. The OX-OsTPS30 plants exhibited resistance to gamma-rays, as compared to WT. The OX-OsTPS30 plants had significantly increased height and weight after gamma irradiation. Additionally, the activity of antioxidant enzymes was increased more in OX OsTPS30 plants than in WT plants after gamma irradiation. Furthermore, the OsTPS30-GFP fusion protein was mostly localized in the chloroplast, suggesting that OsTPS30 is putative MEP pathway-related terpene synthase.

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참고문헌

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