Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Evaluation of the periodontal and pulpal healing of replanted rat molars with doxycycline root conditioning

  • Nam, Ok Hyung (Department of Pediatric Dentistry, Kyung Hee University School of Dentistry) ;
  • Cheon, Kyounga (Department of Pediatric Dentistry, University of Alabama at Birmingham) ;
  • Kim, Mi Sun (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital at Gangdong) ;
  • Lee, Hyo-Seol (Department of Pediatric Dentistry, Kyung Hee University School of Dentistry) ;
  • Choi, Sung Chul (Department of Pediatric Dentistry, Kyung Hee University School of Dentistry)
  • Received : 2019.02.16
  • Accepted : 2019.05.16
  • Published : 2019.06.30
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Abstract

Purpose: This study aimed to evaluate periodontal and pulpal healing in replanted rat teeth, preserved under different storage conditions, with or without root conditioning using doxycycline. Methods: A total of 40 maxillary first molars extracted from 20 Sprague-Dawley rats were stored for different durations under different conditions (5 minutes in dry storage and 60 minutes in Hank's balanced salt solution [HBSS]) and subsequently replanted. The rats were divided into 2 groups based on the use of root surface treatment: the doxycycline group (root surface treated with doxycycline) and the control group (no doxycycline treatment). Eight weeks after replantation, the animals were sacrificed, and the teeth were evaluated using micro-computed tomography (micro-CT) and histomorphometric analysis. Results: In the micro-CT analysis, the doxycycline group showed the same rate of occurrence of periapical radiolucency as was observed in the control group, but a lower degree of root resorption in teeth replanted after 60 minutes of storage in HBSS (P<0.05). In the histomorphometric analysis, the doxycycline group exhibited no improvement in either pulpal or periodontal healing of the replanted tooth after 5 minutes of dry storage, but showed a lower grade of surface root resorption ($1.37{\pm}0.77$) and inflammatory resorption in the teeth stored for 60 minutes in HBSS ($1.33{\pm}0.71$). Conclusions: In conclusion, doxycycline improved the periodontal healing of replanted teeth stored for 60 minutes in HBSS, whereas doxycycline did not improve periodontal healing of replanted tooth after 5 minutes of dry storage. Within the limits of this study, doxycycline showed more favorable periodontal healing despite delayed replantation.

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References

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