Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Novel Chlorella Species with Cold Resistance and High Lipid Accumulation for Biodiesel Production

  • Koh, Hyun Gi (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Kang, Nam Kyu (Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign) ;
  • Kim, Eun Kyung (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Suh, William I. (Advanced Biomass R&D Center (ABC), KAIST) ;
  • Park, Won-Kun (Department of Chemistry and Energy Engineering, Sangmyung University) ;
  • Lee, Bongsoo (Department of Microbial and Nano Materials, College of Science and Technology, Mokwon University) ;
  • Chang, Yong Keun (Advanced Biomass R&D Center (ABC), KAIST)
  • Received : 2019.04.04
  • Accepted : 2019.05.17
  • Published : 2019.06.28
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Abstract

Chlorella spp. are green algae that are found across wide-ranging habitats from deserts to arctic regions, with various strains having adapted to survive under diverse environmental conditions. In this study, two novel Chlorella strains (ABC-002, ABC-008) were isolated from a freshwater lake in South Korea during the winter season and examined for possible use in the biofuel production process. The comparison of ABC-002 and ABC-008 strains with Chlorella vulgaris UTEX265 under two different temperatures ($10^{\circ}C$, $25^{\circ}C$) revealed their cold-tolerant phenotypes as well as high biomass yields. The maximum quantum yields of UTEX25, ABC-002, and ABC-008 at $10^{\circ}C$ were 0.5594, 0.6747, and 0.7150, respectively, providing evidence of the relatively higher cold-resistance capabilities of these two strains. Furthermore, both the biomass yields and lipid content of the two novel strains were found to be higher than those of UTEX265; the overall lipid productivities of ABC-002 and ABC-008 were 1.7 ~ 2.8 fold and 1.6 ~ 4.2 fold higher compared to that of UTEX265, respectively. Thus, the high biomass and lipid productivity over a wide range of temperatures indicate that C. vulgaris ABC-002 and ABC-008 are promising candidates for applications in biofuel productions via outdoor biomass cultivation.

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References

  1. Slocombe SP, Zhang Q, Ross M, Anderson A, Thomas NJ, Lapresa A, et al. 2015. Unlocking nature's treasure-chest: screening for oleaginous algae. Sci. Rep. 5: 9844. https://doi.org/10.1038/srep09844
  2. Mata TM, Martins AA, Caetano NS. 2010. Microalgae for biodiesel production and other applications: a review. Renew. Sust. Energ. Rev. 14: 217-232. https://doi.org/10.1016/j.rser.2009.07.020
  3. Neofotis P, Huang A, Sury K, Chang W, Joseph F, Gabr A, et al. 2016. Characterization and classification of highly productive microalgae strains discovered for biofuel and bioproduct generation. Algal. Res. 15: 164-178. https://doi.org/10.1016/j.algal.2016.01.007
  4. Weyer KM, Bush DR, Darzins A, Willson BD. 2010. Theoretical maximum algal oil production. Bioenerg. Res. 3: 204-213. https://doi.org/10.1007/s12155-009-9046-x
  5. Safi C, Zebib B, Merah O, Pontalier PY, Vaca-Garcia C. 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renew. Sust. Energ. Rev. 35: 265-278. https://doi.org/10.1016/j.rser.2014.04.007
  6. Ahn JW, Hwangbo K, Lee SY, Choi HG, Park YI, Liu JR, et al. 2012. A new Arctic Chlorella species for biodiesel production. Bioresour. Technol. 125: 340-343. https://doi.org/10.1016/j.biortech.2012.09.026
  7. Treves H, Raanan H, Finkel OM, Berkowicz SM, Keren N, Shotland Y, et al. 2013. A newly isolated Chlorella sp. from desert sand crusts exhibits a unique resistance to excess light intensity. Fems Microbiol. Ecol. 86: 373-380. https://doi.org/10.1111/1574-6941.12162
  8. NAABB. 2016. National Algal Biofuels Technology Review. 7.
  9. Kao CY, Chiu SY, Huang TT, Dai L, Wang GH, Tseng CP, et al. 2012. A mutant strain of microalga Chlorella sp. for the carbon dioxide capture from biogas. Biomass Bioenergy 36: 132-140. https://doi.org/10.1016/j.biombioe.2011.10.046
  10. Wang LA, Min M, Li YC, Chen P, Chen YF, Liu YH, et al. 2010. Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl. Biochem. Biotechnol. 162: 1174-1186. https://doi.org/10.1007/s12010-009-8866-7
  11. Del Campo JA, Rodriguez H, Moreno J, Vargas MA, Rivas J, Guerrero MG. 2004. Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta). Appl. Microbiol. Biotechnol. 64: 848-854. https://doi.org/10.1007/s00253-003-1510-5
  12. Kwon S, Kang NK, Koh HG, Shin SE, Lee B, Jeong BR, et al. 2018. Enhancement of biomass and lipid productivity by overexpression of a bZIP transcription factor in Nannochloropsis salina. Biotechnol. Bioeng. 115: 331-340. https://doi.org/10.1002/bit.26465
  13. Ajjawi I, Verruto J, Aqui M, Soriaga LB, Coppersmith J, Kwok K, et al. 2017. Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator. Nat. Biotechnol. 35: 647-652. https://doi.org/10.1038/nbt.3865
  14. Shin WS, Lee B, Kang NK, Kim YU, Jeong WJ, Jeong BR, et al. 2017-MS. Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci. Rep. 7: 17929. https://doi.org/10.1038/s41598-017-18221-0
  15. Shin WS, Lee B, Jeong BR, Chang YK, Kwon JH. 2016. Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity. J. Appl. Phycol. 28: 3193-3202. https://doi.org/10.1007/s10811-016-0874-8
  16. Richardson JW, Johnson MD, Outlaw JL. 2012. Economic comparison of open pond raceways to photo bio-reactors for profitable production of algae for transportation fuels in the Southwest. Algal Res. 1: 93-100. https://doi.org/10.1016/j.algal.2012.04.001
  17. Davis R, Aden A, Pienkos PT. 2011. Techno-economic analysis of autotrophic microalgae for fuel production. Appl. Energ. 88: 3524-3531. https://doi.org/10.1016/j.apenergy.2011.04.018
  18. Richardson JW, Johnson MD, Zhang XZ, Zemke P, Chen W, Hu Q. 2014. A financial assessment of two alternative cultivation systems and their contributions to algae biofuel economic viability. Algal Res. 4: 96-104. https://doi.org/10.1016/j.algal.2013.12.003
  19. Gong M, Bassi A. 2017. Investigation of Chlorella vulgaris UTEX 265 cultivation under light and low temperature stressed conditions for lutein production in flasks and the coiled tree photo-bioreactor (CTPBR). Appl. Biochem. Biotechnol. 183: 652-671. https://doi.org/10.1007/s12010-017-2537-x
  20. Rosenberg JN, Kobayashi N, Barnes A, Noel EA, Betenbaugh MJ, Oyler GA. 2014. Comparative analyses of three Chlorella species in response to light and sugar reveal distinctive lipid accumulation patterns in the microalga C. sorokiniana. PLoS One 9(4): e92460. https://doi.org/10.1371/journal.pone.0092460
  21. Wan MX, Wang RM, Xia JL, Rosenberg JN, Nie ZY, Kobayashi N, et al. 2012. Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures. Biotechnol. Bioeng. 109: 1958-1964. https://doi.org/10.1002/bit.24477
  22. Farooq W, Lee YC, Ryu BG, Kim BH, Kim HS, Choi YE, et al. 2013. Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity. Bioresour. Technol. 132: 230-238. https://doi.org/10.1016/j.biortech.2013.01.034
  23. Lee YC, Lee K, Oh YK. 2015. Recent nanoparticle engineering advances in microalgal cultivation and harvesting processes of biodiesel production: a review. Bioresour. Technol. 184: 63-72. https://doi.org/10.1016/j.biortech.2014.10.145
  24. Kobayashi N, Barnes A, Jensen T, Noel E, Andlay G, Rosenberg JN, et al. 2015. Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales. Bioresour. Technol. 198: 246-255. https://doi.org/10.1016/j.biortech.2015.08.124
  25. Beijerinck M. 1890. Kulturversuche mit Zoochlorellen, Lichenengonidien und anderen niederen Algen. Botanische Ztg. 48: 729.
  26. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, et al. 2008. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54: 621-639. https://doi.org/10.1111/j.1365-313X.2008.03492.x
  27. Teoh ML, Chu WL, Marchant H, Phang SM. 2004. Influence of culture temperature on the growth, biochemical composition and fatty acid profiles of six Antarctic microalgae. J. Appl. Phycol. 16: 421-430. https://doi.org/10.1007/s10811-004-5502-3
  28. Seaburg KG, Parker BC, Wharton RA, Simmons GM. 1981. Temperature-growth responses of algal isolates from antarctic oases. J. Phycology 17: 353-360. https://doi.org/10.1111/j.0022-3646.1981.00353.x
  29. Murchie EH, Lawson T. 2013. Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J. Exp. Bot. 64: 3983-3998. https://doi.org/10.1093/jxb/ert208
  30. Lv HX, Qu G, Qi XZ, Lu LN, Tian CG, Ma YH. 2013. Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation. Genomics 101: 229-237. https://doi.org/10.1016/j.ygeno.2013.01.004
  31. Yao Y, Lu Y, Peng KT, Huang T, Niu YF, Xie WH, et al. 2014. Glycerol and neutral lipid production in the oleaginous marine diatom Phaeodactylum tricornutum promoted by overexpression of glycerol-3-phosphate dehydrogenase. Biotechnol. Biofuels 7: 110. https://doi.org/10.1186/1754-6834-7-110
  32. Nam K, Lee H, Heo SW, Chang YK, Han JI. 2017. Cultivation of Chlorella vulgaris with swine wastewater and potential for algal biodiesel production. J. Appl. Phycol. 29: 1171-1178. https://doi.org/10.1007/s10811-016-0987-0
  33. Bouaid A, Vazquez R, Martinez M, Aracil J. 2016. Effect of free fatty acids contents on biodiesel quality. Pilot plant studies. Fuel 174: 54-62. https://doi.org/10.1016/j.fuel.2016.01.018
  34. Stansell GR, Gray VM, Sym SD. 2012. Microalgal fatty acid composition: implications for biodiesel quality. J. Appl. Phycol. 24: 791-801. https://doi.org/10.1007/s10811-011-9696-x
  35. Jiang HM, Gao KS. 2004. Effects of lowering temperature during culture on the production of polyunsaturated fatty acids in the marine diatom Phaeodactylum tricornutum (Bacillariophyceae). J. Phycology 40: 651-654. https://doi.org/10.1111/j.1529-8817.2004.03112.x
  36. Van Wagenen J, Miller TW, Hobbs S, Hook P, Crowe B, Huesemann M. 2012. Effects of light and temperature on fatty acid production in nannochloropsis salina. Energies 5: 731-740. https://doi.org/10.3390/en5030731
  37. Bamgboye AI, Hansen AC. 2008. Prediction of cetane number of biodiesel fuel from the fatty acid methyl ester (FAME) composition. Int. Agrophys. 22: 21-29.
  38. Ramos MJ, Fernandez CM, Casas A, Rodriguez L, Perez A. 2009. Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour. Technol. 100: 261-268. https://doi.org/10.1016/j.biortech.2008.06.039