Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Application of Dynamic Regulation to Increase L-Phenylalanine Production in Escherichia coli

  • Wu, Jie (School of Biological Engineering, Dalian Polytechnic University) ;
  • Liu, Yongfei (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences) ;
  • Zhao, Sheng (Department of Animal Science, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University) ;
  • Sun, Jibin (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences) ;
  • Jin, Zhaoxia (School of Biological Engineering, Dalian Polytechnic University) ;
  • Zhang, Dawei (Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences)
  • Received : 2019.01.28
  • Accepted : 2019.05.21
  • Published : 2019.06.28
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Abstract

Current strategies of strain improvement processes are mainly focused on enhancing the synthetic pathways of the products. However, excessive metabolic flux often creates metabolic imbalances, which lead to growth retardation and ultimately limit the yield of the product. To solve this problem, we applied a dynamic regulation strategy to produce $\text\tiny{L}$-phenylalanine ($\text\tiny{L}$-Phe) in Escherichia coli. First, we constructed a series of Phe-induced promoters that exhibited different strengths through modification of the promoter region of tyrP. Then, two engineered promoters were separately introduced into a Phe-producing strain xllp1 to dynamically control the expression level of one pathway enzyme AroK. Batch fermentation results of the strain xllp3 showed that the titer of Phe reached 61.3 g/l at 48 h, representing a titer of 1.36-fold of the strain xllp1 (45.0 g/l). Moreover, the $\text\tiny{L}$-Phe yields on glucose of xllp3 (0.22 g/g) were also greatly improved, with an increase of 1.22-fold in comparison with the xllp1 (0.18 g/g). In summary, we successfully improved the titer of Phe by using dynamic regulation of one key enzyme and this strategy can be applied for improving the performance of strains producing other aromatic amino acids and derived compounds.

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References

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