Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Nitrifying-genes Dynamics in the Enriched Bacterial Consortium Inoculated with Humic Soil

부식토 유래 질산화세균 consortium의 질산화 유전자 거동 특성

  • Seo, Yoon-Joo (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Lee, Yun-Yeong (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Choi, Hyung-Joo (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • 서윤주 (이화여자대학교환경공학과) ;
  • 이윤영 (이화여자대학교환경공학과) ;
  • 최형주 (이화여자대학교환경공학과) ;
  • 조경숙 (이화여자대학교환경공학과)
  • Received : 2018.09.25
  • Accepted : 2018.10.26
  • Published : 2019.06.28
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Abstract

In this study, the effects of ammonium concentration ($117.5-1155.0mg-N{\cdot}l^{-1}$), nitrite concentration ($0-50.0mg-N{\cdot}l^{-1}$), and temperature ($15-35^{\circ}C$) on nitrification performance and its functional genes (amoA-arc, amoA-bac, hao) in an enriched consortium inoculated with humic acid were determined. Notably, the maximum nitrification rate value was observed at $315mg-N{\cdot}l^{-1}$ of ammonium, but the highest functional gene copy numbers were obtained at $630mg-N{\cdot}l^{-1}$ of ammonium. No inhibition of the nitrification rate and functional gene copy numbers was observed via the added nitrites. The optimum temperature for maximum nitrification performance was observed to be $30^{\circ}C$. The amoA-bac copy numbers were also greater than those of amoA-arc under all test conditions. Notably, amoA-arc copy numbers and nitrification efficiency showed a positive relationship in network analysis. These results indicate that ammonium-oxidizing archaea and bacteria play important roles in the nitrification process.

Keywords

References

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