Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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A Study of Antibiotic Resistance of Enterococci Isolated from Recycling Water of Floor Fountains in Gwangju Area

광주지역내 바닥분수 재이용수에서 분리한 장구균의 항생제 내성 연구

  • Kim, Yeon-Hee (Health and Environment Research Institute of Gwangju) ;
  • Song, Hyeong-myeong (Health and Environment Research Institute of Gwangju) ;
  • Choi, Yeong-Seop (Health and Environment Research Institute of Gwangju) ;
  • Lee, Yoon-Kook (Health and Environment Research Institute of Gwangju) ;
  • Park, Jin-Hwan (Health and Environment Research Institute of Gwangju) ;
  • Kim, Sun-Jung (Health and Environment Research Institute of Gwangju) ;
  • Kim, Ha-Ram (Health and Environment Research Institute of Gwangju) ;
  • Kang, Yu-Mi (Health and Environment Research Institute of Gwangju) ;
  • Bae, Seok-Jin (Health and Environment Research Institute of Gwangju) ;
  • Cho, Yong-Gwan (Health and Environment Research Institute of Gwangju)
  • 김연희 (광주광역시보건환경연구원) ;
  • 송형명 (광주광역시보건환경연구원) ;
  • 최영섭 (광주광역시보건환경연구원) ;
  • 이윤국 (광주광역시보건환경연구원) ;
  • 박진환 (광주광역시보건환경연구원) ;
  • 김선정 (광주광역시보건환경연구원) ;
  • 김하람 (광주광역시보건환경연구원) ;
  • 강유미 (광주광역시보건환경연구원) ;
  • 배석진 (광주광역시보건환경연구원) ;
  • 조영관 (광주광역시보건환경연구원)
  • Received : 2019.04.30
  • Accepted : 2019.06.10
  • Published : 2019.06.30
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Abstract

Objectives: This study aims to evaluate water quality in terms of microorganisms and identify the antibiotic resistance of Enterococci isolated from the recycling water in floor fountains at three parks and one reservoir in the Gwangju area. Methods: Water samples were analyzed for Enterococci using membrane Enterococcus indoxyl ${\beta}$ d glucoside agar (mEI) as described in USEPA Method 1600. The vancomycin-resistant Enterococci with VanA and VanB were identified by PCR. An examination of the antibiotic resistance of isolates against 14 antibiotics was performed by the disk diffusion method. Results: The drinking water quality criterion was exceeded for total colony counts in 68% of all recycling water samples. The average concentration of total califorms and fecal coliforms was 139,325 and 413 CFU/100 mL, respectively. VanA and VanB were not detected from the isolates. We found the antibiotic resistant Enterococci strains to be E. faecalis, E. faecium, E. durans, E. mundtii, E. hirae, and E. thailandicus. The isolates were resistant to Rifampin (50%), Erythromycin (25.8%), Tetracycline (10.2%), Nitrofurantoin (8.1%), Minocycline (3.1%), Erythromycin (1.2%), Penicillin (0.7%), Norfloxacin (0.5%), and Teicoplanin (0.5%) among the 14 antibiotics tested. Antibiotic resistance tests for Enterococci from the recycling water of floor fountains resulted in 30.2% showing resistance to two or more antibiotics. Conclusions: These results showed that the multi-antibiotic resistance of Enterococci, E. coli, and others should be investigated continuously in each environment field.

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