한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제32권3호
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  • Pages.208-215
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  • 2019
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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택란의 일반성분, 지방산 조성 및 세포독성 효과

Proximate Analysis, Fatty Acid Composition of Lycopus lucidus Turcz. and Its Cytotoxic Effect in Cancer Cell Lines

  • 나은 (한국해양대학교 해양과학기술전문대학원 대학원) ;
  • 이정우 (한국해양대학교 해양생명과학부 대학원) ;
  • 임선영 (한국해양대학교 해양생명과학부)
  • Na, Eun (Ocean Science and Technology School, Korea Maritime & Ocean University) ;
  • Lee, Jung-Woo (Division of Marine Bioscience, Korea Maritime & Ocean University) ;
  • Lim, Sun-Young (Division of Marine Bioscience, Korea Maritime & Ocean University)
  • 투고 : 2019.04.04
  • 심사 : 2019.05.14
  • 발행 : 2019.06.30
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초록

In this paper, we investigate to determine quality characteristics, fatty acid composition and cytotoxic effect of extracts and fractions from whole Lycopus lucidus Turcz. roots. Additionally, we evaluated cytotoxic activity against the growth of human fibrosarcoma cells (HT-1080) and human gastric adenocarcinoma (AGS), human colon cancer cell (HT-29) lines using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acetone+methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. were obtained through solvent extraction. Then we further fractionated both extracts with n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water. In fatty acid composition, L. lucidus Turcz. contained 33.2% of 18:1n-9 and 1.81% of 18:3n-3, respectively. The incorporation of treatment with A+M and MeOH extracts and n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water fractions dose-dependently increased cytotoxicity against the growth of HT-1080 and AGS, HT-29 cancer cells (p<0.05). The A+M extract had a higher inhibitory effect on the growth of all cancer cells in comparison to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions showed a higher inhibitory effect after proliferating the three cancer cells. These results suggest that the 85% aq. MeOH and n-hexane fractions have a potential to inhibit the growth of human cancer cell lines.

키워드

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Fig. 1. Effect of acetone/methylene chloride (A+M) and methanol (MeOH) extract from L. lucidus Turcz. on the cell viability of HT-1080 human fibrosarcoma cells.

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Fig. 2. Effect of solvent fractions from L. lucidus Turcz. on the cell viability of HT-1080 human fibrosarcoma cells.

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Fig. 3. Effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz.

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Fig. 4. Effect of solvent fractions from L. lucidus Turcz. on the cell viability of AGS human gastric adenocarcinoma cells.

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Fig. 5. Effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. on the cell viability of HT-29 human colon cancer cells.

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Fig. 6. Effect of solvent fractions from L. lucidus Turcz. on the cell viability of AGS human gastric adenocarcinoma cells.

Table 1. Several fatty acid compositions (% area) of Lycopus lucidus Turcz

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