Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-oxidant activity of avicularin and isovitexin from Lespedeza cuneata

  • Lee, Ju Sung (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Ah Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Quilantang, Norman G. (Department of Plant Science and Technology, Chung-Ang University) ;
  • Geraldino, Paul John L. (Department of Biology, University of San Carlos) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2019.03.04
  • Accepted : 2019.04.09
  • Published : 2019.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study evaluated the anti-oxidant activity of avicularin and isovitexin from Lespedeza cuneata using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) scavenging assays. The results showed that among the four fractions, the highest OH radical scavenging activity was exhibited by the n-butanol fraction. The DPPH radical scavenging activities of avicularin and isovitexin were higher than 50% at a concentration of $100{\mu}g/mL$. Moreover, one hundred micrograms per liter of avicularin and isovitexin exhibited effective anti-oxidant activity, with the OH radical scavenging rates being 87.54 and 91.48%, respectively. These results suggest that Lespedeza cuneata would be useful anti-oxidant agents from natural sources.

Keywords

References

  1. Holliwell B, Gutteridge JM, Cross CE (1992) Free radicals anti-oxidants, and human disease: Where are we now? J Lab Clin Med 119: 598-620
  2. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D (1993) Dietary anti-oxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet 342: 1007-1011 https://doi.org/10.1016/0140-6736(93)92876-U
  3. Yoo G, Park SJ, Lee TH, Yang H, Baek Y-S, Kim N, Kim YJ, Kim SH (2015) Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharidestimulated BV-2 microglia cells. Pharmacogn Mag 11: 651-656 https://doi.org/10.4103/0973-1296.160466
  4. Kim HY, Ko JY, Song SB, Kim JI, Seo HI, Lee JS, Kwak DY, Jung TW, Kim KY, Oh IS, Jeong HS, Woo KS (2012) Anti-oxidant and aglucosidase inhibition activities of solvent fractions from methanolic extract of Sericea lespedeza (Lespedeza cuneata G. Don). J Korean Soc Food Sci Nutr 41: 1508-1514 https://doi.org/10.3746/jkfn.2012.41.11.1508
  5. Nam J-S, Park S-Y, Jang H-L, Rhee YH (2017) Phenolic compounds in different parts of young Annona muricata cultivated in Korea and their anti-oxidant activity. Appl Biol Chem 60: 535-543 https://doi.org/10.1007/s13765-017-0309-5
  6. Lee J, Rodriguez JP, Lee KH, Park JY, Kang KS, Hahm D-H, Huh CK, Lee SC, Lee S (2017) Determination of flavonoids from Cirsium japonicum var. maackii and their inhibitory activities against aldose reductase. Appl Biol Chem 60: 487-496 https://doi.org/10.1007/s13765-017-0302-z
  7. Williams RJ, Spencer JP, Rice-Evans C (2014) Flavonoids: anti-oxidants or signalling molecules? Free Radic Biol Med 36: 838-849 https://doi.org/10.1016/j.freeradbiomed.2004.01.001
  8. Choi JS, Cho CS, Kim CJ (2010) Cytoprotective effect of Lespedeza cuneata extract on glucose toxicity. J Korean Oriental Med 31: 79-100
  9. Lee H, Jung JY, Hwangbo M, Ku SK, Kim YW, Jee SY (2013) Antiinflammatory effects of Lespedeza cuneata in vivo and in vitro. Kor J Herbol 28: 83-92
  10. Kim MS, Sharma BR, Rhyu DY (2016) Beneficial Effect of Lespedeza cuneata (G. Don) water extract on streptozoticin-induced type 1 diabetes and cytokine-induced beta-cell damage. Nat Prod Sci 22: 175-179 https://doi.org/10.20307/nps.2016.22.3.175
  11. Kwon DJ, Kim JK, Ham YH, Bae YS (2007) Flavone glycosides from the aerial parts of Lespedeza cuneata G. Don. J Korean Soc Appl Biol Chem 50: 344-347
  12. Kwon DJ, Bae YS (2009) Flavonoids from the aerial parts of Lespedeza cuneata. Biochem Syst Ecol 37: 46-48 https://doi.org/10.1016/j.bse.2008.11.014
  13. Kim SM, Kang KS, Jho EH, Jung YJ, Nho CW, Um BH, Pan CH (2011) Hepatoprotective effect of flavonoid glycosides from Lespedeza cuneata against oxidative stress induced by tert-butyl hyperoxide. Phytother Res 25: 1011-1017 https://doi.org/10.1002/ptr.3387
  14. Kim JA, Jung YS, Kim MY, Yang SY, Lee S, Kim YH (2011) Protective effect of components isolated from Lindera erythrocarpa against oxidative stress-induced apoptosis of H9c2 cardiomyocytes. Phytother Res 25: 1612-1617 https://doi.org/10.1002/ptr.3465
  15. He M, Min J-W, Kong W-L, He X-H, Li J-X, Peng B-W (2016) A review on the pharmacological effects of vitexin and isovitexin. Fitoterapia 115: 74-85 https://doi.org/10.1016/j.fitote.2016.09.011
  16. Kwon A-R, Oh M-M, Paek K-Y, Park S-Y (2018) The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis 'Spring Dancer'. Hort Environ Biotechnol 59: 179-188 https://doi.org/10.1007/s13580-018-0018-y
  17. Nam T-G, Lim YJ, Eom SH (2018) Flavonoid accumulation in common buckwheat (Fagopyrum esculentum) sprout tissues in response to light. Hort Environ Biotechnol 59: 19-27 https://doi.org/10.1007/s13580-018-0003-5
  18. Baek JW, Lee TK, Song J-H, Choi E, Ko H-J, Lee S, Choi SU, Lee S, Yoo S-W, Kim S-H, Kim KH (2018)Lignan glycosides and flavonoid glycosides from the aerial portion of Lespedeza cuneata and their biological evaluations. Molecules 23: 1920-1931 https://doi.org/10.3390/molecules23081920
  19. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T (1989) Effects of the interaction of tannins with coexisting substances, VI. Effects of tannins and related polyphenols on superoxide anion radical and on 1,1-diphenyl-2-pricrylhydrazyl radical. Chem Pharm Bull 37: 2016-2021 https://doi.org/10.1248/cpb.37.2016
  20. Chung SK, Osawa T, Kawakishi S (1997) Hydroxyl radical-scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61: 118-123 https://doi.org/10.1271/bbb.61.118
  21. Aruoma OI (1994) Nutrition and health aspects of free radicals and antioxidant. Food Chem Toxicol 62: 671-683 https://doi.org/10.1016/0278-6915(94)90011-6
  22. Kim JS, Kim MJ (2010) In vitro anti-oxidant activity of Lespedeza cuneata methanolic extracts. J Med Plants Res 2: 674-679
  23. Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid anti-oxidant: chemistry metabolism and structure-activity relationships. J Nutr Biochem 13: 572-584 https://doi.org/10.1016/S0955-2863(02)00208-5
  24. Seyoum A, Asres K, Kandeel Ei-Fiky F (2006) Structure-radical scavenging activity relationships of flavonoids. Phytochemistry 67: 2058-2070 https://doi.org/10.1016/j.phytochem.2006.07.002

Cited by

  1. Sericea lespedeza (Lespedeza juncea var. sericea) for sustainable small ruminant production: Feed, helminth suppressant and meat preservation capabilities vol.270, 2019, https://doi.org/10.1016/j.anifeedsci.2020.114688
  2. Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method vol.64, pp.1, 2021, https://doi.org/10.3839/jabc.2021.010
  3. Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses vol.10, pp.10, 2021, https://doi.org/10.3390/antiox10101615