Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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3-week repeated dose oral toxicity study of Clausena excavata extract in ICR mice

ICR 마우스에서 핑크왐피 추출물의 3주간 반복 투여 독성 연구

  • Park, Ju-Hyoung (College of Pharmacy, Dankook University) ;
  • Cho, Young-Rak (Bio-center, Gyeonggido Business and Science Accelerator) ;
  • Kim, Young Min (Bio-center, Gyeonggido Business and Science Accelerator) ;
  • Kang, Jae-Shin (Biological Genetic Resources Utilization Division, National Institute of Biological Resources (NIBR)) ;
  • Oh, Joa Sub (College of Pharmacy, Dankook University) ;
  • Ahn, Eun-Kyung (Bio-center, Gyeonggido Business and Science Accelerator)
  • Received : 2018.12.24
  • Accepted : 2019.02.28
  • Published : 2019.06.30
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Abstract

Medicinal herb in Asian countries has been traditionally used for a long time. However, the safety and adverse effect of medicinal herb have not been established yet. The aim of this study is to evaluate toxicity in the oral administration of Clausena excavata (C. excavata) in male ICR mice for 3 weeks and the noobserved adverse effect level (NOAEL). C. excavata has been used as a medicinal herb for the treatment of dermatopathy, malaria, abdominal pain, dysentery, and enteritis. C. excavata was orally administered daily for 21 days at a dose of 100, 250, 500, 1000, and 2000 mg/kg/day (MPK). There were no significant differences in mortalities, clinical signs, body weight changes, hematological, and serum biochemistry examination in all animals administrated with C. excavate. Consequently, these findings indicated that C. excavata did not affect the toxic effect in ICR mice and the NOAEL of C. excavata was considered as more than 2000 MPK.

핑크왐피는 피부병, 말라리아, 복통, 이질 및 장염 치료를 위한 약용 식물로 사용되고 있다. 본 연구의 목적은 핑크왐피의 안전성을 확인하기 위하여, 수컷 ICR 마우스를 이용하여 핑크왐피를 3주 반복 경구 투여하여 최대무독성용량을 평가했다. 핑크왐피를 100, 250, 500, 1000, 및 2000 mg/kg으로 투여한 결과, 모든 시험물질 투여군에서 이상이 관찰되지 않았다. 사망률, 임상 증상, 체중 변화, 혈액 학적 검사 및 혈청 생화학 검사에서 유의적인 차이는 없었고, 경미한 변동은 핑크왐피의 투여로 인한 영향이 아닌 정상 범위 내에서의 변화로 간주 되어진다. 결과적으로, 핑크왐피는 모든 시험물질 투여군에서 독성 영향을 미치지 않았으며, 핑크왐피의 최대무독성용량은 2000 mg/kg 이상으로 간주되었다.

Keywords

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